Distribution of virulence associated genes in avian pathogenic Escherichia coli isolates from Tamil Nadu

The present study describes the distribution of 4 virulence associated genes in 25 avian pathogenic Escherichia coli (APEC) isolates recovered from poultry affected with colibacillosis. Serotypes (12) were ecountered when these isolates were serotyped and O1, O24, O53, O60, O78 and O91 were the pred...

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Bibliographic Details
Published in:Indian journal of animal sciences 2006-04, Vol.76 (4)
Main Authors: Thangapandian, E, Vijayarani, K, Ramadass, P, Nainar, A.M
Format: Article
Language:English
Subjects:
AVES DE CORRAL
COLIBACILLOSE
COLIBACILLOSIS
COLIBACILOSIS
ESCHERICHIA COLI
http://www.fao.org/aos/agrovoc#c_21534
http://www.fao.org/aos/agrovoc#c_33700
http://www.fao.org/aos/agrovoc#c_5629
http://www.fao.org/aos/agrovoc#c_6145
http://www.fao.org/aos/agrovoc#c_7598
PATHOGENICITY
PATOGENICIDAD
POULTRY
POUVOIR PATHOGENE
TAMIL NADU
VOLAILLE
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Summary:The present study describes the distribution of 4 virulence associated genes in 25 avian pathogenic Escherichia coli (APEC) isolates recovered from poultry affected with colibacillosis. Serotypes (12) were ecountered when these isolates were serotyped and O1, O24, O53, O60, O78 and O91 were the predominent serotypes. Multiplex polymerase chain reaction (PCR) was done to detect the presence of virulence associated genes like iss, fumC, iucD and tsh. One or other target genes was present in 22 of the 25 isolates while the remaining 3 isolates did not have any. Among the 22 positive isolates, only 4 had all the 4 genes. The frequency of iss, fimC, fmC, iucD and tsh was 88, 56, 20 and 16;, respectively. Intra serotypic variation, with isolates of same serotypes possessing different virulence genes was also observed among the serotypes O1, O53, O60 and O91. Multiplex PCR was very useful in rapidly identifying pathogenic E.coli isolates.
ISSN:0367-8318