Change in electrophoretic profile of porcine spermatozoal proteins during epididymal transit

Mammalian spermatozoa acquire their motility and ability to fertilize during their transit through epididymis and are characterized with functional changes in sperm membrane proteins. The present study was undertaken to electrophoretically characterize proteins in epididymal and testicular sperms an...

Full description

Saved in:
Bibliographic Details
Published in:Indian journal of animal sciences 2008-05, Vol.78 (5)
Main Authors: Mohan, N.H, Sarmah, B.C, Tamuli, M.K, Das, Anubrata, Bujarbaruah, K.M
Format: Article
Language:English
Subjects:
CAPRIN
CAPRINOS
ESPERMATOZOO
GOATS
http://www.fao.org/aos/agrovoc#c_3324
http://www.fao.org/aos/agrovoc#c_7287
http://www.fao.org/aos/agrovoc#c_7682
SPERMATOZOA
SPERMATOZOIDE
TESTES
TESTICULE
TESTICULOS
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mammalian spermatozoa acquire their motility and ability to fertilize during their transit through epididymis and are characterized with functional changes in sperm membrane proteins. The present study was undertaken to electrophoretically characterize proteins in epididymal and testicular sperms and cauda epididymal fluid to understand spermatozoal maturation process in porcine testes. The number of protein bands in testicular, epididymal spermatozoa and epididymal fluid were 22, 17 and 17 respectively. The proteins having molecular weight (MW) of 105, 97, 89, 79, 73, 64, 32, 29, 24, 22, 20 and 18 kilodaltons, which were present in the testicular spermatozoa could not be identified in the epididymal sperms. On the other hand, the epididymal spermatozoa expressed proteins having MW of 102, 93, 82, 66, 62, 55, 28 and 21 kilodaltons, which were absent in testicular spermatozoa. The epididymal fluid shared proteins having MW of 55, 52, 42 and 39 kilodaltons with testicular and epididymal spermatozoa. The elucidation of physiological events related to protein modification, appearance and masking require further studies.
ISSN:0367-8318