Sequences of the N-termini of coat proteins of Portoguese Zucchini yellow mosaic virus isolates and of an epitope recognized by a monoclonal antibody [Cucurbita pepo L.]

The sequences of the N-terminal 132 nucleotides of the coat protein genes of eleven Portuguese isolates of Zucchini yellow mosaic virus (ZYMV) were determined. The nucleotide sequence similarities among the eleven isolates suggested two groups, based on different codon usage for the amino acid resid...

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Published in:Journal of plant pathology 2005-11, Vol.87 (3)
Main Authors: Cardoso, F.H. (Instituto National de Engenharia, Tecnologia e Inovacao, Lisboa (Portugal). Departamento de Biotecnologia), Armada, A. (Instituto National de Engenharia, Tecnologia e Inovacao, Lisboa (Portugal). Departamento de Biotecnologia), Fonseca, A.M. (Universidade do Algarve, Faro (Portugal). Campus de Gambelas), Santos, M.T. (Estacao Agronómica Nacional, Oeiras (Portugal)), Sequeira, J. (Estacao Agronómica Nacional, Oeiras (Portugal)), Clemente, A. (Instituto National de Engenharia, Tecnologia e Inovacao, Lisboa (Portugal). Departamento de Biotecnologia), Sequeira, O. (Estacao Agronómica Nacional, Oeiras (Portugal)), Novo, C. (Instituto National de Engenharia, Tecnologia e Inovacao, Lisboa (Portugal). Departamento de Biotecnologia)
Format: Article
Language:English
Subjects:
amino acid sequences
anticorpi monoclonali
Anticorps monoclonal
Anticuerpos monoclonales
cucurbita pepo
elisa
genes
geni
Gène
Identificación
identification
identificazione
monoclonal antibodies
nucleotide sequence
pcr
portogallo
portugal
potyvirus del mosaico giallo dello zucchino
Potyvirus mosaico amarill calabacín
Potyvirus mosaïque jaune courgette
Secuencia nucleotídica
Secuencias de aminoácidos
sequenza nucleotidica
sequenze aminoacidiche
Séquence d'acides aminés
Séquence nucléotidique
Test elisa
zucchini yellow mosaic potyvirus
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Summary:The sequences of the N-terminal 132 nucleotides of the coat protein genes of eleven Portuguese isolates of Zucchini yellow mosaic virus (ZYMV) were determined. The nucleotide sequence similarities among the eleven isolates suggested two groups, based on different codon usage for the amino acid residues in seven positions. Comparisons among the encoded amino acid sequences and those previously reported indicated that in this region the Portuguese isolates were identical to isolates from the USA (California), Germany, Italy and France. A monoclonal antibody was produced against a Portuguese isolate that recognized the epitope SGTQPT and cross-reacted with particles of other potyviruses [Sono state determinate le sequenze di 132 nucleotidi terminali N dei geni delle proteine capsidiche di 11 isolati portoghesi del Virus del mosaico giallo dello zucchino (ZYMV). Le somiglianze delle sequenze nucleotidiche nell´ambito degli 11 isolati hanno fatto ipotizzare due gruppi, in base all´uso diverso dei codoni per i residui di aminoacidi in 7 posizioni. I confronti fra le sequenze codificate degli aminoacidi e quelle riportate in precedenza hanno indicato che in questa regione gli isolati portoghesi sono simili a quelli provenienti da California (USA), Germania, Italia e Francia. E´ stato prodotto un anticorpo monoclonale per un isolato portoghese che ha riconosciuto l´epitopo SGTQPT e ha espresso una reazione crociata con particelle di altri Potyvirus.]
ISSN:1125-4653