Analysis of differentially regulated proteins in TM4 cells treated with bisphenol A

BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-06, Vol.68 (6), p.1201-1208
Main Authors: Lee, D.Y. (Korea Univ., Seoul (Korea R.)), Lee, S.S, Joo, W.A, Lee, E.J, Kim, C.W
Format: Article
Language:English
Subjects:
2D gel electrohphoresis
ANALYTICAL METHODS
Animals
Benzhydryl Compounds
Biological and medical sciences
bisphenol A
calcium binding protein
CALCIUM BINDING PROTEINS
Calcium-Binding Proteins - genetics
Cell Line
Cell Shape
Cell Survival
CELLS
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling - methods
Gene Expression Regulation - drug effects
Heat-Shock Proteins - genetics
HSP 27
Male
matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)
MICE
PHENOLIC COMPOUNDS
Phenols - pharmacology
PLASTICS
PROTEINS
Sertoli Cells - drug effects
Sertoli Cells - metabolism
SPERMATOGENESIS
TM 4 cell
TOXICITY
VIABILITY
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Summary:BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the effect of BPA on protein expression in TM4 cells (a mouse Sertoli cell line) known to play an essential role in Spermatogenesis was investigated by two-dimensional electrophoresis (2-DE). After 16 h exposure to 50, 100, 150, 200, and 250 μM of BPA, the viability of TM4 cells decreased to about 90, 85, 78, 55, and 30% of control respectively. Approximately 800 protein spots in TM4 cells were analyzed by 2-DE with pH 4-7 linear immobilized pH gradient (IPG) Dry Strip, and 11 proteins which showed significantly different expression levels were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Among these, HSP 27 and placental calcium binding protein may be proteins differentially expressed by BPA exposure.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.68.1201