Analysis of differentially regulated proteins in TM4 cells treated with bisphenol A

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-06, Vol.68 (6), p.1201-1208
• Main Authors: Lee, D.Y. (Korea Univ., Seoul (Korea R.)), Lee, S.S, Joo, W.A, Lee, E.J, Kim, C.W
• Format: Article
• Language: English
• Subjects: 2D gel electrohphoresis; ANALYTICAL METHODS; Animals; Benzhydryl Compounds; Biological and medical sciences; bisphenol A; calcium binding protein; CALCIUM BINDING PROTEINS; Calcium-Binding Proteins - genetics; Cell Line; Cell Shape; Cell Survival; CELLS; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - methods; Gene Expression Regulation - drug effects; Heat-Shock Proteins - genetics; HSP 27; Male; matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS); MICE; PHENOLIC COMPOUNDS; Phenols - pharmacology; PLASTICS; PROTEINS; Sertoli Cells - drug effects; Sertoli Cells - metabolism; SPERMATOGENESIS; TM 4 cell; TOXICITY; VIABILITY
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.68.1201

BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the effect of BPA on protein expression in TM4 cells (a mouse Sertoli cell line) known to play an essential role in Spermatogenesis was investigated by two-dimensional electrophoresis (2-DE). After 16 h exposure to 50, 100, 150, 200, and 250 μM of BPA, the viability of TM4 cells decreased to about 90, 85, 78, 55, and 30% of control respectively. Approximately 800 protein spots in TM4 cells were analyzed by 2-DE with pH 4-7 linear immobilized pH gradient (IPG) Dry Strip, and 11 proteins which showed significantly different expression levels were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Among these, HSP 27 and placental calcium binding protein may be proteins differentially expressed by BPA exposure.