Gene transfection into HeLa cells by vesicles containing cationic peptide lipid

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-08, Vol.69 (8), p.1453-1458
• Main Authors: hama, Y. (Ehime Univ., Matsuyama (Japan). Coll. of Agriculture), Heike, Y, Sugahara, T, Sakata, K, Yoshimura, N, Hisaeda, Y, Hosokawa, M, Takashima, S, Kato, K
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Blood; cationic lipid vesicle; Cationic peptides; CATIONS; CELLS; Culture Media; Cytotoxicity; Drugs; Fundamental and applied biological sciences. Psychology; gene transfection; Gene transfer; GENES; HeLa cell; HeLa Cells; Humans; LIPIDS; Lipids - chemistry; lipofection; luciferase; Lysine; microcapsules; PEPTIDES; Peptides - chemistry; Plasmids; Poly-L-lysine; Q1; sorbitan; TRANSFECTION; Vesicles
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.69.1453

Lipid vesicles are potentially useful as microcapsules for drug and/or gene delivery. We developed cationic lipid vesicles consisting mainly of sorbitan monooleate (Span 80) and cationic peptide lipid (CPL), and evaluated the CPL vesicles as gene transfection vectors. The optimum CPL concentration for gene transfection into HeLa cells was found to be 20 wt % of total lipid, and such CPL vesicles did not exhibit significant cytotoxidty. Co-culture of Poly-L-lysine and plasmids prior to making CPL vesicle-plasmid complexes was effective. Lipofection using LipofectAMINE was suppressed in 10% serum-supplemented medium. The transfection efficiency of 20 wt % CPL vesicles, however, was not affected by serum in the medium when plasmids were treated with poly-L-lysine.