Permeability of mouse oocytes and embryos at various development stages to five cryoprotectants

To assess the permeability of mouse oocytes and embryos, matured oocytes and embryos at various stages of development were placed in five cryoprotectant solutions at 25 C for 25 min. From the cross-sectional areas of the oocytes/embryos, the relative change in volume was analyzed. In oocytes, shrink...

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Bibliographic Details
Published in:The Journal of reproduction and development 2005-04, Vol.51 (2)
Main Authors: Pedro, P.B.(Kochi Univ., Nankoku (Japan). Faculty of Agriculture), Yokoyama, E, Zhu, S.E, Yoshida, N, Valdez, D.M.Jr, Tanaka, M, Edashige, K, Kasai, M
Format: Article
Language:English
Subjects:
ANIMAL EMBRYOS
CRIOPROTECTORES
CRYOPROTECTANTS
CRYOPROTECTEUR
DEVELOPMENTAL STAGES
EMBRIONES ANIMALES
EMBRYON ANIMAL
ETAPAS DE DESARROLLO
MICE
OVA
OVULE
OVULO
PERMEABILIDAD
PERMEABILITE
PERMEABILITY
RATON
SOURIS
STADE DE DEVELOPPEMENT
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Summary:To assess the permeability of mouse oocytes and embryos, matured oocytes and embryos at various stages of development were placed in five cryoprotectant solutions at 25 C for 25 min. From the cross-sectional areas of the oocytes/embryos, the relative change in volume was analyzed. In oocytes, shrinkage was least extensive and recovery was quickest in the propylene glycol solution, showing that propylene glycol permeates the oocytes most rapidly. Dimethyl sulfoxide, acetamide, and ethylene glycol permeated the oocytes slightly more slowly than propylene glycol. The oocytes in glycerol shrunk extensively and then expanded marginally, indicating slow permeation. The volume changes of 1-cell and 2-cell embryos were similar to those of oocytes, showing little change in permeability. In 8-cell embryos, the volume recovered much faster than in the earlier stages especially in glycerol and acetamide. In morulae, the volume recovery was much faster in glycerol and in ethylene glycol; in ethylene glycol, the extent of shrinkage was small and the recovery was fast, indicating an extremely rapid permeation. Although the permeability of oocytes/embryos generally increased as embryo development proceeded, the degree of increase varied greatly among the cryoprotectants, Interestingly, the volume change in propylene glycol was virtually unaffected by the stage of development. Such information will be valuable for determining a suitable protocol for the cryopreservation of oocytes/embryos at different stages of development.
ISSN:0916-8818