Aggregated form of dextransucrases from Leuconostoc mesenteroides NRRL B-512F and its constitutive mutant

Purified dextransucrases [EC 2.4.1.5], DSW-D and DSW-G, from Leuconostoc mesenteroides B-512F were obtained from affinity chromatography with DEAE-Sephadex A-50 by elution with clinical dextran and guanidine-HCl, respectively, DSM-G was purified from the B-512F mutant strain SH 3002, which produces...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995, Vol.59 (5), p.776-780
Main Authors: Funane, K. (National Food Research Inst., Tsukuba, Ibaraki (Japan)), Yamada, M, Shiraiwa, M, Takahara, H, Yamamoto, N, Ichishima, E, Kobayashi, M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteriology
BIOCENOSE
BIOCENOSIS
BIOCOENOSIS
Biological and medical sciences
Biotechnology
DEXTRANAS
DEXTRANE
DEXTRANS
Dextrans - chemistry
FRUCTOFURANOSIDASA
FRUCTOFURANOSIDASE
Fundamental and applied biological sciences. Psychology
Glucosyltransferases - chemistry
Glucosyltransferases - genetics
Glucosyltransferases - isolation & purification
LEUCONOSTOC
Leuconostoc - chemistry
Leuconostoc - enzymology
Leuconostoc - genetics
Leuconostoc mesenteroides
Metabolism. Enzymes
Microbiology
Molecular Sequence Data
MUTANT
MUTANTES
MUTANTS
Sequence Homology, Amino Acid
Streptococcus - enzymology
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Summary:Purified dextransucrases [EC 2.4.1.5], DSW-D and DSW-G, from Leuconostoc mesenteroides B-512F were obtained from affinity chromatography with DEAE-Sephadex A-50 by elution with clinical dextran and guanidine-HCl, respectively, DSM-G was purified from the B-512F mutant strain SH 3002, which produces dextransucrase constitutively. Although the sugar contents of the purified enzymes were different, their molecular masses by SDS-PAGE were all 170kDa. DSW-D and DSW-G were highly aggregated and the all the activities were eluted at the void volume (V 0 ) on Sepharose 6B, while the DSM-G was eluted at 1.2 × V 0 volume. On rechromatography, DSM-G was separated into three peaks corresponding to the aggregated form, monomeric form, and partially digested form, respectively. The aggregation of Leuconostoc dextransucrase was looser than that of streptococcal glucosyltransferases, but the structures of these enzymes had high homology with each other.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.59.776