Molecular Cloning and Expression of a Novel Cuticle Protein Gene from the Chinese Oak Silkmoth, Antheraea pernyi

In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The Ap...

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Published in:International Journal of Industrial Entomology 2005-03, Vol.10 (1)
Main Authors: Kim, B.Y. (Miryang National University, Miryang, Republic of Korea), Park, N.S. (Miryang National University, Miryang, Republic of Korea), Jin, B.R. (Dong-A University, Busan, Republic of Korea), Kang, P.D. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea), Lee, B.H. (Korea University, Seoul, Republic of Korea), Seong, S.I. (The University of Suwon, Suwon, Republic of Korea), Hwang, J.S. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea), Chang, J.S. (Daejin University, Pocheon, Republic of Korea), Lee, S.M. (Miryang National University, Miryang, Republic of Korea), E-mail: serilsm@mnu.ac.kr
Format: Article
Language:English
Subjects:
ANTHERAEA PERNYI
cDNA
chinese oak silkmoth
Cuticle protein
INSECTA
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Summary:In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B (43% protein sequence identity), followed by Locusta migratoria-76 (42% protein sequence identity).
ISSN:1598-3579