Molecular Characterization of A Novel Bacillus thuringiensis Strain from China

A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella was isolated from a dust sample collected from Chinese tobacco warehouse and characterized. The isolate named B. thuringiensis LY-99 was determined to belong to subsp. alesti (H3a3c) by an H antisera ag...

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Bibliographic Details
Published in:International Journal of Industrial Entomology 2005-09, Vol.11 (1)
Main Authors: Qi, Xu Feng (Huazhong Agricultural University, Wuhan, P. R. China), Li, Ming Shun (Seoul National University, Seoul, Republic of Korea), Choi, J.Y. (Seoul National University, Seoul, Republic of Korea), Kim, Y.S. (Seoul National University, Seoul, Republic of Korea), Wang, Y. (Seoul National University, Seoul, Republic of Korea), Kang, J.N. (Seoul National University, Seoul, Republic of Korea), Choi, H.K. (Seoul National University, Seoul, Republic of Korea), Je, Y.H. (Seoul National University, Seoul, Republic of Korea), Song, Ji Zhen (China National Tobacco Corporation, Zhengzhou, P. R. China), Li, Jian Hong (Huazhong Agricultural University, Wuhan, P. R. China), E-mail: jianhl@mail.hzau.edu.cn
Format: Article
Language:English
Subjects:
Bacillus thuringiensis LY-99
Bacillus thuringiensis subsp. alesti
cry gene
Insecticidal activity
PLUTELLA XYLOSTELLA
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Summary:A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella was isolated from a dust sample collected from Chinese tobacco warehouse and characterized. The isolate named B. thuringiensis LY-99 was determined to belong to subsp. alesti (H3a3c) by an H antisera agglutination test and produced bipyramidal inclusions. Plasmid and crystal protein patterns of the LY-99 were different from those of the reference strain, subsp. alesti. PCR analysis with specific primers revealed that this isolate contained abundant cry genes including cry1Aa, cry1Ac, cry1B, cry1D, cry1E, cry1F and cry2 genes, which was absolutely different from cry gene profile of the subsp. alesti.
ISSN:1598-3579