Genetic Transformation of Ajuga multiflora Bunge with Agrobacterium rhizogenes and 20-Hydroxyecdysone Production in Hairy Roots

An efficient transformation system for Ajuga multiflora Bunge was established by using Agrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 2...

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Published in:Journal of plant biology = Singmul Hakhoe chi 2005-06, Vol.48 (2)
Main Authors: Kim, O.T. (Chonnam National University, Gwangju, Republic of Korea), Manickavasagm, M. (Bharathidasan University Tiruchirapalli, Tamil Nadu, India), Kim, Y.J. (Chonnam National University, Gwangju, Republic of Korea), Jin, M.R. (Chonnam National University, Gwangju, Republic of Korea), Kim, K.S. (National Institute of Crop Science, RDA, Muan, Republic of Korea), Seong, N.S. (National Institute of Crop Science, RDA, Suwon, Republic of Korea), Hwang, B. (Chonnam National University, Gwangju, Republic of Korea), E-mail: bhwang@chonnam.ac.kr
Format: Article
Language:English
Subjects:
20-hydroxyecdysone
AGROBACTERIUM RHIZOGENES
Ajuga multiflora
hairy roots
Ri-plasmid
rol genes
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Summary:An efficient transformation system for Ajuga multiflora Bunge was established by using Agrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 20-hydroxyecdysone (20-HE). PCR amplification of rooting locus (rol) genes revealed that the left hand-transferred DNA of the root-inducing plasmid was inserted into the genome of our transformed Ajuga hairy roots.
ISSN:1226-9239
1867-0725
DOI:10.1007/BF03030416