Adaptation of a local wild infectious bursal disease virus on chicken embryo fibroblast cell culture

A local very virulent infectious bursal disease virus (vvIBDV) was successfully adapted to grown on chicken embryo fibroblast (CEF) cell culture with concurrent loss of pathogenicity in specific pathogen free (SPF) chicks. The IBDV was isolated from field outbreaks and identified through agar gel pr...

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Bibliographic Details
Published in:International journal of agriculture and biology 2007-11, Vol.9 (6), p.925-927
Main Authors: Khan, M.A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Veterinary Microbiology), Hussain, I. (University of Agriculture, Faisalabad (Pakistan). Dept. of Veterinary Microbiology), Siddique, M, Rahman, S.U, Arshad, M
Format: Article
Language:English
Subjects:
ADAPTABILITY
ANIMAL EMBRYOS
CELL CULTURE
CHICKENS
FIBROBLASTS
HAEMAGGLUTINATION TESTS
INFECTIOUS BURSAL DISEASE VIRUS
PATHOGENICITY
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Summary:A local very virulent infectious bursal disease virus (vvIBDV) was successfully adapted to grown on chicken embryo fibroblast (CEF) cell culture with concurrent loss of pathogenicity in specific pathogen free (SPF) chicks. The IBDV was isolated from field outbreaks and identified through agar gel precipitation test (AGPT) and counter current immmoelectrophoresis (CCIE) using specific antiserum. The positive samples (Bursae) were pooled and IBDV was purified through chloroform treatment and sucrose gradient centrifugation technique. The chick embryo infectivity (EID50) of the purified vvIBDV was 10(4.84) mL-1. The virus was then passaged on CEF cell culture. It was completely adapted to grow in CEF cell culture after thirteenth passage. With reverse passive haemagglutination (RPHA), the titre of adapted virus was found as 1:1024 at fifteenth passage with complete loss of pathogenicity.
ISSN:1560-8530
1814-9596