Occurrence of aflatoxins in maize grains from Central Areas of Punjab, Pakistan

Mycotoxins (MTs) are natural contaminants of food and feed chiefly produced by moulds of genera Penicillium, Aspergillus, and Fusarium and are toxic to humans and animals. Aflatoxins are toxic metabolites produced mainly by Aspergillus flavus, parasiticus and nomius Aflatoxin B1 (AFB1) is a potent c...

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Published in:International journal of agriculture and biology 2010-07, Vol.12 (4), p.571-575
Main Authors: Ahsan, S. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry and Biochemistry), Bhatti, I.A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry and Biochemistry), Asi, M.R. (Nuclear Inst. for Agriculture and Biology, Faisalabad (Pakistan)), Bhatti, H.N, Sheikh, M.A
Format: Article
Language:English
Subjects:
AFLATOXINS
CONTAMINATION
EXTRACTION
FLOURS
HPLC
MAIZE
PAKISTAN
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Summary:Mycotoxins (MTs) are natural contaminants of food and feed chiefly produced by moulds of genera Penicillium, Aspergillus, and Fusarium and are toxic to humans and animals. Aflatoxins are toxic metabolites produced mainly by Aspergillus flavus, parasiticus and nomius Aflatoxin B1 (AFB1) is a potent carcinogen, teratogen and mutagen. Forty samples of maize grains were collected randomly from urban, semi-urban and rural areas. These were ground to 20 mesh and extracted using 100 mL mixture of acetonitrile and water (84:16). The filtered extract was cleaned up with MycoSep # 226 column. The presence of aflatoxins (B1, B2, G1 & G2) was determined by using reverse-phase high performance liquid chromatography (RP-HPLC) using fluorescent detection system. Out of 40 maize samples, 34 were found contaminated with aflatoxins.
ISSN:1560-8530
1814-9596