Interactions of tumor cells with vascular endothelial cell monolayers: a model for metastatic invasion [Calf bovine aortic endothelial cells]

The interactions of tumorigenic and nontumorigenic human and rodent cells with vascular endothelial cells and their underlying extracellular matrix were studied in culture. The abilities of various cells to attach to endothelial monolayers and cause morphologic changes, such as rupture of endothelia...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1979-11, Vol.76 (11), p.5704-5708
Main Authors: Kramer, Randall H., Nicolson, Garth L.
Format: Article
Language:English
Subjects:
Animals
Blood cells
Blood Vessels - pathology
Cattle
Cell Adhesion
Cell lines
Cell Movement
Cells, Cultured
Endothelial cells
Endothelium - pathology
Extracellular matrix
Extracellular Space - physiology
Intercellular junctions
Intercellular Junctions - pathology
Lymphocytes
Melanoma
Microscopy, Electron
Microscopy, Electron, Scanning
Neoplasm Metastasis
Neoplasms, Experimental - pathology
Tumor cell line
Tumors
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Summary:The interactions of tumorigenic and nontumorigenic human and rodent cells with vascular endothelial cells and their underlying extracellular matrix were studied in culture. The abilities of various cells to attach to endothelial monolayers and cause morphologic changes, such as rupture of endothelial-endothelial cell interactions leading to retraction of endothelial cells and exposure of extracellular matrix, as well as their propensities to invade and underlap retracted endothelial monolayers and continue migration were assessed by time-lapse and phase-contrast microscopy as well as scanning and transmission electron microscopy. In general, highly malignant or highly invasive cells in vivo were capable of attachment, invasion, and migration under endothelial cells in vitro. This system may be useful for elucidating mechanisms of tumor cell arrest and extravasation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.76.11.5704