Autolysis of lactococci: detection of lytic enzymes by polyacrylamide gel electrophoresis and characterization in buffer systems

• Published in: Applied and Environmental Microbiology 1995-10, Vol.61 (10), p.3598-3603
• Main Authors: ØSTLIE, H. M, VEGARUD, G, LANGSRUD, T
• Format: Article
• Language: English
• Subjects: amide hydrolase; amide hydrolases; amido hidrolasa; Bacteria; Bacteriology; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; electroforesis; electrophorese; electrophoresis; Enzymes; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; hidrolasas; hydrolase; hydrolases; Lactococcus; lactococcus lactis; Micrococcus luteus; Mission oriented research; peptidasas; peptidase; peptidases; Physiology and metabolism
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.61.10.3598-3603.1995

Lactococcal strains were screened for bacteriolytic activity against Micrococcus luteus cells, lactococcal cells, and cell walls. Thirty strains were screened for bacteriolytic activity against cells and cell walls incorporated into agar medium. Enzymes from all strains hydrolyzed the substrates; however, the activity against Micrococcus cells was much higher than against Lactococcus cells or cell walls. Electrophoretic profiles of bacteriolytic activities of culture supernatants, sodium dodecyl sulfate-treated cell extracts, cell wall fractions, and cell extracts were analyzed in sodium dodecyl sulfate-polyacrylamide gels containing M. luteus cells or lactococcal cell walls as the substrate. The 22 strains tested contained two to five lytic bands in the culture supernatant, ranging in size between 32 and 53 kDa. The cell extracts, the sodium dodecyl sulfate-treated cell extracts, and the cell wall fractions revealed two lytic bands of 47 and 53 kDa. Effects of external factors on autolysis of some strains were determined in buffer systems. Optimal autolysis was observed in the exponential growth phase at pH 6.0 to 7.5 and at a temperature of 30 degrees C. Two of three strains tested seemed to contain a glycosidase, and all three strains contained an N-acetylmuramyl-L-alanine amidase or an endopeptidase.