Effect of antioxidant vitamin supplementation on DNA damage and repair in human lymphoblastid cells

In this study the possible protective effects of ascorbic acid and alpha-tocopherol (singly and in combination) on Raji lymphoblastoid cells exposed to various doses of X-rays or hydrogen peroxide (H2O2) are investigated. DNA strand breaks and alkali-labile sites were measured using the alkaline com...

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Bibliographic Details
Published in:Nutrition and cancer 1997, Vol.27 (2)
Main Authors: Sweetman, S.F, Strain, J.J, McKelvey-Martin, V.J
Format: Article
Language:English
Subjects:
ACIDE ASCORBIQUE
ACIDO ASCORBICO
ADN
ANTIOXIDANTES
ANTIOXIDANTS
ANTIOXYDANT
ASCORBIC ACID
CELL CULTURE
CELLS
CELLULE
CELULAS
COMPLEMENT ALIMENTAIRE
CULTIVO DE CELULAS
CULTURE DE CELLULE
DAMAGE
DANOS
DEGAT
DNA
DNA REPAIR
DOSAGE EFFECTS
EFECTOS DE DOSIFICACION
EFFET DOSE
FREQUENCY
GLICOSILTRANSFERASAS
GLYCOSYLTRANSFERASE
GLYCOSYLTRANSFERASES
HYDROGEN PEROXIDE
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
MUTANT
MUTANTES
MUTANTS
PEROXIDO DE HIDROGENO
PEROXYDE D'HYDROGENE
PROTECTION
RAYON X
RAYOS-X
SINERGISMO
SUPERVIVENCIA
SUPLEMENTOS
SUPPLEMENTS
SURVIE
SURVIVAL
SYNERGISM
SYNERGISME
TOCOFEROLES
TOCOPHEROL
TOCOPHEROLS
VITAMINAS
VITAMINE
VITAMINS
X RAYS
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Summary:In this study the possible protective effects of ascorbic acid and alpha-tocopherol (singly and in combination) on Raji lymphoblastoid cells exposed to various doses of X-rays or hydrogen peroxide (H2O2) are investigated. DNA strand breaks and alkali-labile sites were measured using the alkaline comet assay. Survival and hypoxanthine guanine phosphoribosyl transferase mutant frequency were measured using the colony-forming assay. Ascorbic acid (60 micromolar) and alpha-tocopherol (30 micromolar) were added singly or together to cell culture medium 24 hours before treatment and were present during treatment. After the 24-hour supplementation period with ascorbic acid alone, alpha-tocopherol alone, and ascorbic acid + alpha-tocopherol, the level of endogenous DNA damage was significantly lower (p 0.05) than in the nonsupplemented culture, as assessed by the comet assay. By use of the comet assay, it was observed that ascorbic acid exhibited an overall protective effect against DNA damage induced after X-ray treatment, whereas alpha-tocopherol exhibited an overall protective effect against DNA damage induced after H2O2 treatment. Significant increases were observed in the percent survival after 1-Gy X-rays and 5 and 20 micromolar H2O2 in those cultures supplemented with ascorbic acid alone and alpha-tocopherol alone relative to the nonsupplemented cultures. The endogenous level of mutant frequency, was also significantly decreased in the presence of ascorbic acid relative to the nonsupplemented culture. These findings are consistent with the concept that ascorbic acid alpha-tocopherol can, under certain conditions, protect against oxidatively induced DNA damage
ISSN:0163-5581
1532-7914