Binding of Fe(OH)3 to lactobacillus delbrueckii sp. bulgaricus and Lactobacillus acidophilus: apparent role of hydrogen peroxide and free radicals

Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus acidophilus, both used extensively as probiotics and to produce fermented milk products, were investigated for their ability to bind Fe-(OH)3 as a function of time in the presence and absence of approximately 200 micromolar H2O2 and in the...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1997-03, Vol.45 (3)
Main Authors: Kot, E, Furmanov, S, Bezkorovainy, A
Format: Article
Language:English
Subjects:
FER
FERMENTED MILK PRODUCTS
FISICA
FREE RADICALS
HIDROXIDOS
HIERRO
HYDROGEN PEROXIDE
HYDROXIDES
HYDROXYDE
IRON
LACTOBACILLUS ACIDOPHILUS
LACTOBACILLUS DELBRUECKII
MILK PRODUCTS
PEROXIDO DE HIDROGENO
PEROXYDE D'HYDROGENE
PHYSICS
PHYSIQUE
PROBIOTICOS
PROBIOTICS
PROBIOTIQUE
PRODUCTOS LACTEOS
PRODUIT LAITIER
RADICAL LIBRE
RADICALES LIBRES
THERMODYNAMICS
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Summary:Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus acidophilus, both used extensively as probiotics and to produce fermented milk products, were investigated for their ability to bind Fe-(OH)3 as a function of time in the presence and absence of approximately 200 micromolar H2O2 and in the presence and absence of glucose. There was a very rapid initial phase, followed by a second slow phase, and, when fermentable sugar was absent, a third rapid phase. Thermodynamic parameters were determined for the initial rapid binding phase using Scatchard plots. The binding process was entropy driven against an enthalpy barrier, indicating that hydrophobic-type bonding was involved. Results were similar for both species of lactobacilli, and in the presence or absence of glucose and/or H2O2. In the later phases, binding of Fe(OH)3 was decreased if carried out without H2O2 in the medium. Free radicals, apparently produced from H2O2 and iron, were able to alter cell surfaces to allow for greater binding of Fe(OH)3. The ability of probiotics to bind Fe(OH)3 may serve to limit the availability of iron to pathogenic microorganisms
ISSN:0021-8561
1520-5118