Kappa-casein polymorphisms in indian dairy cattle and buffalo: a new genetic variant in buffalo

We screened 57 Sahiwal cattle (Bos indicus) and 53 Murrah, 19 Nili-Ravi and 11 Egyptian buffaloes (Bubalus bubalis) to detect the polymorphisms at kappa-casein (CSN3) gene using the polymerase chain reaction (PCR). CSN3 A and B alleles were identified by PCR-RFLP using the restriction enzymes that d...

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Bibliographic Details
Published in:Animal biotechnology 1998-01, Vol.9 (2), p.81-87
Main Authors: Mitra, A. (Institut fuer Tierwissenschaften der TU Munchen, Freising-Weihenstephan, Germany.), Schlee, P, Krause, I, Blusch, J, Werner, T, Balakrishnan, C.R, Pirchner, F
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Animals
Bos indicus
BOVIN
Bubalus bubalis
Buffaloes - genetics
Caseins - genetics
CATTLE
Cattle - genetics
GANADO BOVINO
GENETIC POLYMORPHISM
INDE
INDIA
Milk - chemistry
Molecular Sequence Data
POLIMORFISMO GENETICO
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
POLYMORPHISME GENETIQUE
Sequence Analysis, DNA
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Summary:We screened 57 Sahiwal cattle (Bos indicus) and 53 Murrah, 19 Nili-Ravi and 11 Egyptian buffaloes (Bubalus bubalis) to detect the polymorphisms at kappa-casein (CSN3) gene using the polymerase chain reaction (PCR). CSN3 A and B alleles were identified by PCR-RFLP using the restriction enzymes that detect the underlying nucleotide changes at codon 136 (TaqI) and at codon 148 (HindIII or HinfI) in cattle. The frequency of CSN3 B allele in the Sahiwal cattle was estimated as 0.16 with no homozygous BB animal. Using the same set of primers as used in the Sahiwal cattle, a part of exon IV of buffalo CSN3 gene was amplified, but restriction enzyme analysis using HindIII/HinfI and TaqI did not reveal any polymorphism. However, DNA sequencing of the amplified fragment (GenBank Acc. No. U96662) revealed one polymorphism at codon 135 (ThrACC ?IleATC) in buffalo; the frequencies of 135 Thr/Ile alleles were estimated as 0.88 and 0.12, respectively.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495399809525896