DNA sequence similarity between California isolates of Cryptosporidium parvum

We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence ide...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1998-04, Vol.64 (4), p.1584-1586
Main Authors: Pereira, M. das G.C. (University of California, Davis, Tulare, CA.), Atwill, E.R, Crawford, M.R, LeFebvre, R.B
Format: Article
Language:English
Subjects:
Animals
Animals, Domestic - parasitology
Animals, Wild - parasitology
Base Sequence
Biological and medical sciences
CALIFORNIA
CALIFORNIE
Cattle - parasitology
CRYPTOSPORIDIUM PARVUM
Cryptosporidium parvum - classification
Cryptosporidium parvum - genetics
Cryptosporidium parvum - isolation & purification
Deoxyribonucleic acid
DNA
DNA Primers - genetics
DNA, Protozoan - genetics
DNA, Protozoan - isolation & purification
Environmental and Public Health Microbiology
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Goats - parasitology
Horses - parasitology
Humans
Microbiology
MOLECULAR SEQUENCE DATA
NUCLEOTIDE SEQUENCE
Parasites
Polymerase Chain Reaction
Protozoa
SECUENCIA NUCLEOTIDICA
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEOTIDIQUE
Sewage - parasitology
Species Specificity
Swine - parasitology
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Description
Summary:We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel. Am. J. Trop. Med. Hyg. 45:688-694,1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.64.4.1584-1586.1998