Antibiotic Resistances of Starter and Probiotic Strains of Lactic Acid Bacteria

The antibiotic resistances of 45 lactic acid bacteria strains belonging to the genera Lactobacillus, Streptococcus, Lactococcus, Pediococcus, and Leuconostoc were investigated. The objective was to determine antibiotic resistances and to verify these at the genetic level, as is currently suggested b...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2007-02, Vol.73 (3), p.730-739
Main Authors: Hummel, Anja S, Hertel, Christian, Holzapfel, Wilhelm H, Franz, Charles M.A.P
Format: Article
Language:English
Subjects:
Acids
Amino Acid Sequence
Animals
Anti-Bacterial Agents - pharmacology
Bacterial Proteins - genetics
Biological and medical sciences
Cheese - microbiology
DNA Gyrase - chemistry
DNA Gyrase - genetics
DNA Topoisomerase IV - chemistry
DNA Topoisomerase IV - genetics
Drug resistance
Drug Resistance, Bacterial - genetics
Food Microbiology
Fundamental and applied biological sciences. Psychology
Lactobacillus
Lactobacillus - drug effects
Lactobacillus - genetics
Lactobacillus salivarius
Lactococcus
Leuconostoc
Meat Products - microbiology
Microbial Sensitivity Tests - methods
Microbial Sensitivity Tests - standards
Microbiology
Molecular Sequence Data
Pediococcus
Penicillin
Probiotics
Sequence Analysis, DNA
Streptococcaceae - classification
Streptococcaceae - drug effects
Streptococcaceae - genetics
Streptococcus
Studies
Yogurt - microbiology
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Summary:The antibiotic resistances of 45 lactic acid bacteria strains belonging to the genera Lactobacillus, Streptococcus, Lactococcus, Pediococcus, and Leuconostoc were investigated. The objective was to determine antibiotic resistances and to verify these at the genetic level, as is currently suggested by the European "qualified presumption of safety" safety evaluation system for industrial starter strains. In addition, we sought to pinpoint possible problems in resistance determinations. Primers were used to PCR amplify genes involved in β-lactam antibiotic, chloramphenicol, tetracycline, and erythromycin resistance. The presence of ribosomal protection protein genes and the ermB gene was also determined by using a gene probe. Generally, the incidences of erythromycin, chloramphenicol, tetracycline, or β-lactam resistances in this study were low (
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.02105-06