Arthrobacter aurescens TCl atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli

Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Esc...

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Bibliographic Details
Published in:Applied and environmental microbiology 2004, Vol.70 (7), p.4402-4407
Main Authors: Sajjaphan, K, Shapir, N, Wackett, L.P, Palmer, M, Blackmon, B, Tomkins, J, Sadowsky, M.J
Format: Article
Language:English
Subjects:
Arthrobacter aurescens
atrazine
bacterial artificial chromosomes
biodegradation
genes
linkage (genetics)
metabolism
molecular sequence data
nucleotide sequences
plasmids
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Summary:Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.
ISSN:0099-2240
1098-5336