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Arthrobacter aurescens TCl atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli
Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Esc...
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Published in: | Applied and environmental microbiology 2004, Vol.70 (7), p.4402-4407 |
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creator | Sajjaphan, K Shapir, N Wackett, L.P Palmer, M Blackmon, B Tomkins, J Sadowsky, M.J |
description | Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1. |
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The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. 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Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.</description><subject>Arthrobacter aurescens</subject><subject>atrazine</subject><subject>bacterial artificial chromosomes</subject><subject>biodegradation</subject><subject>genes</subject><subject>linkage (genetics)</subject><subject>metabolism</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>plasmids</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFj01Ow0AMhUcIJMLPGfABiOT8NCRLiIpYsaGsK3fqJEOHiWRPNzkHB2YisWfz_PT8yda7MFmBXZtvqqq5NBli1-VlWeO1uVH9QsQamzYzP88SJ5kPZCML0FlYLQeFXe-BotDiAoOlSIfZO_2GkQMrRFneH9N-eUkajqvrgYTBu3DiI8wBCIoG85Pz6bYyCI9uTVc4ccM52JgC8uACbNVOLM5OjsCmP3fmaiCvfP83b83D63bXv-UDzXsaxen-86PEokqt6qe23lT_E7-p61KX</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Sajjaphan, K</creator><creator>Shapir, N</creator><creator>Wackett, L.P</creator><creator>Palmer, M</creator><creator>Blackmon, B</creator><creator>Tomkins, J</creator><creator>Sadowsky, M.J</creator><scope>FBQ</scope></search><sort><creationdate>2004</creationdate><title>Arthrobacter aurescens TCl atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli</title><author>Sajjaphan, K ; Shapir, N ; Wackett, L.P ; Palmer, M ; Blackmon, B ; Tomkins, J ; Sadowsky, M.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-fao_agris_US2013009478453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Arthrobacter aurescens</topic><topic>atrazine</topic><topic>bacterial artificial chromosomes</topic><topic>biodegradation</topic><topic>genes</topic><topic>linkage (genetics)</topic><topic>metabolism</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>plasmids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sajjaphan, K</creatorcontrib><creatorcontrib>Shapir, N</creatorcontrib><creatorcontrib>Wackett, L.P</creatorcontrib><creatorcontrib>Palmer, M</creatorcontrib><creatorcontrib>Blackmon, B</creatorcontrib><creatorcontrib>Tomkins, J</creatorcontrib><creatorcontrib>Sadowsky, M.J</creatorcontrib><collection>AGRIS</collection><jtitle>Applied and environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sajjaphan, K</au><au>Shapir, N</au><au>Wackett, L.P</au><au>Palmer, M</au><au>Blackmon, B</au><au>Tomkins, J</au><au>Sadowsky, M.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arthrobacter aurescens TCl atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli</atitle><jtitle>Applied and environmental microbiology</jtitle><date>2004</date><risdate>2004</risdate><volume>70</volume><issue>7</issue><spage>4402</spage><epage>4407</epage><pages>4402-4407</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><abstract>Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.</abstract></addata></record> |
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subjects | Arthrobacter aurescens atrazine bacterial artificial chromosomes biodegradation genes linkage (genetics) metabolism molecular sequence data nucleotide sequences plasmids |
title | Arthrobacter aurescens TCl atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli |
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