Activator of Spo(min)::LUC1/WRINKLED1 of Arabidopsis thaliana transactivates sugar-inducible promoters

We isolated an enhancer activation-tagged mutant of Arabidopsis thaliana line sGsL carrying the luciferase (LUC) gene under control of a short sugar-inducible promoter derived from a sweet potato sporamin gene (Spo [superscript min]) that showed high level expression of LUC under non-inducing condit...

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Bibliographic Details
Published in:Plant and cell physiology 2005-04, Vol.46 (4), p.547-556
Main Authors: Masaki, T.(Nagoya Univ. (Japan)), Mitsui, N, Tsukagoshi, H, Nishii, T, Morikami, A, Nakamura, K
Format: Article
Language:English
Subjects:
ACTIVADOR GENICO
ACTIVATEUR GENIQUE
apetala2 protein
ARABIDOPSIS THALIANA
beta-amylase
enhancer elements
EXPRESION GENICA
EXPRESSION DES GENES
GENE ACTIVATORS
GENE EXPRESSION
gene overexpression
GRAINE
luciferase
METABOLISM
METABOLISME
METABOLISMO
molecular sequence data
nucleotide sequences
plant proteins
potatoes
promoter regions
reporter genes
SACCHAROSE
SEEDS
SEMILLA
Solanum tuberosum
sporamin
SUCROSA
SUCROSE
sugars
transcription factors
transcriptional activation
transgenic plants
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Summary:We isolated an enhancer activation-tagged mutant of Arabidopsis thaliana line sGsL carrying the luciferase (LUC) gene under control of a short sugar-inducible promoter derived from a sweet potato sporamin gene (Spo [superscript min]) that showed high level expression of LUC under non-inducing conditions. The activator of Spo [superscript min] ::LUC1 (ASML1) gene located downstream of the enhancer encoded an APETALA2 (AP2)-type AP2 domain protein, and this gene was shown recently to be responsible for the wrinkled1 mutation which causes defective accumulation of seed storage oil. Overexpression of ASML1 cDNA in sGsL plants resulted in enhanced expression of not only the LUC reporter but also endogenous sugar-inducible genes including At{szligbeta}-Amy encoding {szligbeta}-amylase. Transient co-expression of 35S::ASML1 with Spo [superscript min] ::LUC or At{szligbeta}-Amy::LUC reporters in protoplasts resulted in an approximately 10-fold transactivation of LUC expression. This transactivation was lost when the C-terminal acidic region of ASML1 was deleted. Expression of ASML1 was high in reproductive organs, and ASML1 mRNA showed transient accumulation in leaves after treatment with 6% sucrose, whereas it did not respond to abscisic acid. These results suggest that ASML1/WRI1 is a transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pci072