Identification of Variable-Number Tandem-Repeat Loci in Leptospira interrogans Sensu Stricto

Leptospira interrogans sensu stricto is responsible for the most frequent and severe cases of human leptospirosis. The epidemiology and clinical features of leptospirosis are usually associated with the serovars and serogroups of LEPTOSPIRA: Because of the difficulties associated with serological id...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-02, Vol.43 (2), p.539-545
Main Authors: Majed, Z, Bellenger, E, Postic, D, Pourcel, C, Baranton, G, Picardeau, M
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Bacteriology
Base Sequence
Biological and medical sciences
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
Humans
Infectious diseases
Leptospira interrogans - classification
Leptospira interrogans - genetics
Leptospirosis - epidemiology
Leptospirosis - microbiology
Medical sciences
Microbiology
Minisatellite Repeats - genetics
Miscellaneous
Molecular Sequence Data
Polymerase Chain Reaction - methods
Sequence Analysis, DNA
Serotyping
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Summary:Leptospira interrogans sensu stricto is responsible for the most frequent and severe cases of human leptospirosis. The epidemiology and clinical features of leptospirosis are usually associated with the serovars and serogroups of LEPTOSPIRA: Because of the difficulties associated with serological identification of Leptospira strains, we evaluated a novel PCR-based method for typing L. interrogans serovars. Based upon the genome sequence of L. interrogans serovar Lai type strain 5660, 44 loci were analyzed by PCR for their variability in size due to the presence of variable-number tandem repeats (VNTR). Seven VNTR loci were found to be powerful markers for serovar identification, epidemiology, and phylogenetic studies of L. interrogans. This rapid and easy method should greatly contribute to a better knowledge of the epidemiology of LEPTOSPIRA:
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.2.539-545.2005