thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type 1 diabetes

• Published in: European journal of clinical nutrition 2005-05, Vol.59 (5), p.727-730
• Main Authors: Leger, C.L, Carbonneau, M.A, Michel, F, Mas, E, Monnier, L, Cristol, J.P, Descomps, B
• Format: Article
• Language: English
• Subjects: antioxidant activity; antioxidants; atherogenesis; blood chemistry; cardioprotective effect; food processing wastes; humans; hydroxytyrosol; insulin-dependent diabetes mellitus; lipid peroxidation; men; nutritional intervention; olive oil; phenolic compounds; seed extracts; thrombosis; thromboxanes; tyrosine
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/sj.ejcn.1602133

Objective: To assess the antioxidant/non-antioxidant effects of a hydroxytyrosol (HT)-rich phenolic extract from olive mill wastewaters administered with a breakfast. Design, setting and subjects: Five type I diabetic patients received 25 mg of HT the first day and 12.5 mg/day the following 3 days. Blood sampling was carried out at T0 (baseline) and T4d just before the breakfast + HT administration and at time points 1, 2, 3 and 4 h after T0. Urines (24-h) were collected from T0 to T4d. Baseline HbA1c was generally inferior to 10%, glycemia was within the range 6-24 mmol/l, whereas total cholesterol, HDL-chol and triglycerides were normal. Results: The major finding was the 46% decrease in the serum TXB2 production after blood clotting at T4d. Plasma vitamin A, E, beta-carotene were not changed. Vitamin C tended to increase (P=0.075). Plasma antioxidant capacity was enhanced at T0+1 h only, whereas its main determinants (albumin, bilirubin, uric acid) were not modified. Urinary 8-isoPGF2alpha levels were highly variable and were not affected significantly by HT administration. Conclusion: The major effect of HT accounts for an antiaggregating platelet action, leading to a possible prevention of thrombotic and microthrombotic processes.