Achievements in and future prospects for molecular diagnostics of plant-parasitic nematodes

Molecular diagnostics of plant-parasitic nematodes and fundamental research concerning the pathogenicity of these pests have been revolutionized by the polymerase chain reaction. Molecular diagnostics based on sequence divergence between recognized taxa is now well established as a generic approach...

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Bibliographic Details
Published in:Canadian journal of plant pathology 2005-06, Vol.27 (2), p.176-185
Main Author: Blok, V.C
Format: Article
Language:English
Subjects:
AFLP, amplified fragment length polymorphism
CO, cytochrome oxidase
disease diagnosis
ETS, external transcribed spacer
IGS, intergenic spacer
indirect detection
ITS, internal transcribed spacer
literature reviews
mtDNA, mitochondrial DNA
PCN, potato cyst nematode
PCR, polymerase chain reaction
plant diseases and disorders
plant parasitic nematodes
polymerase chain reaction
PPN, plant-parasitic nematode
quantification
RAPD, random amplified polymorphic region
rDNA, ribosomal DNA
ribosomal DNA
RKN, root knot nematode
SCAR, sequence characterized amplified region
sensitivity
sequence analysis
SNP, single nucleotide polymorphism
TRV, Tobacco rattle virus
VNTRs, variable number of tandem repeats
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Summary:Molecular diagnostics of plant-parasitic nematodes and fundamental research concerning the pathogenicity of these pests have been revolutionized by the polymerase chain reaction. Molecular diagnostics based on sequence divergence between recognized taxa is now well established as a generic approach in plant pathology. Technological advances driven largely by medical diagnostics will continue to improve the accuracy, speed, throughput, affordability, and availability of these tools. Utilizing molecular diagnostics effectively for predictive risk assessment, quarantine purposes, and fundamental nematology will continue to require specialized understanding of these plant-parasitic nematodes and their hosts.
ISSN:0706-0661
1715-2992
DOI:10.1080/07060660509507214