Detection and Serotyping of Streptococcus pneumoniae from Nasopharyngeal Samples by PCR-Based Multiplex Assay

We developed a multiplex PCR-based methodology for nasopharyngeal samples maintained in egg thioglycolate antibiotic and skim milk-tryptone-glucose-glycerol media to identify and serotype the most important serotypes of Streptococcus pneumoniae that cause invasive disease in children. This technique...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-12, Vol.43 (12), p.6152-6154
Main Authors: Moreno, Jaime, Hernández, Elkin, Sanabria, Olga, Castañeda, Elizabeth
Format: Article
Language:English
Subjects:
Bacteriological Techniques
Bacteriology
Biological and medical sciences
Child, Preschool
Culture Media
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
Humans
Infant
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Nasopharynx - microbiology
Pneumococcal Infections - microbiology
Polymerase Chain Reaction - methods
Serotyping
Streptococcus pneumoniae
Streptococcus pneumoniae - classification
Streptococcus pneumoniae - genetics
Streptococcus pneumoniae - growth & development
Streptococcus pneumoniae - isolation & purification
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Summary:We developed a multiplex PCR-based methodology for nasopharyngeal samples maintained in egg thioglycolate antibiotic and skim milk-tryptone-glucose-glycerol media to identify and serotype the most important serotypes of Streptococcus pneumoniae that cause invasive disease in children. This technique can be used to study the epidemiology of pneumococcal colonization and the effect of conjugate vaccines.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.43.12.6152-6154.2005