Real-Time PCR Assays for Detection of Bocavirus in Human Specimens

The recently discovered human bocavirus (HBoV) is the first member of the family Parvoviridae, genus Bocavirus, to be potentially associated with human disease. Several studies have identified HBoV in respiratory specimens from children with acute respiratory disease, but the full spectrum of clinic...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Clinical Microbiology 2006-09, Vol.44 (9), p.3231-3235
Main Authors: Lu, Xiaoyan, Chittaganpitch, Malinee, Olsen, Sonja J, Mackay, Ian M, Sloots, Theo P, Fry, Alicia M, Erdman, Dean D
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
Humans
Infectious diseases
Medical sciences
Microbiology
Nucleocapsid Proteins - genetics
Parvoviridae
Parvoviridae Infections - virology
Parvovirinae - genetics
Parvovirinae - isolation & purification
Pneumonia, Viral - virology
Polymerase Chain Reaction - methods
Reproducibility of Results
Sensitivity and Specificity
Taq Polymerase
Thailand
Viral Nonstructural Proteins - genetics
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The recently discovered human bocavirus (HBoV) is the first member of the family Parvoviridae, genus Bocavirus, to be potentially associated with human disease. Several studies have identified HBoV in respiratory specimens from children with acute respiratory disease, but the full spectrum of clinical disease and the epidemiology of HBoV infection remain unclear. The availability of rapid and reliable molecular diagnostics would therefore aid future studies of this novel virus. To address this, we developed two sensitive and specific real-time TaqMan PCR assays that target the HBoV NS1 and NP-1 genes. Both assays could reproducibly detect 10 copies of a recombinant DNA plasmid containing a partial region of the HBoV genome, with a dynamic range of 8 log units (10¹ to 10⁸ copies). Eight blinded clinical specimen extracts positive for HBoV by an independent PCR assay were positive by both real-time assays. Among 1,178 NP swabs collected from hospitalized pneumonia patients in Sa Kaeo Province, Thailand, 53 (4.5%) were reproducibly positive for HBoV by one or both targets. Our data confirm the possible association of HBoV infection with pneumonia and demonstrate the utility of these real-time PCR assays for HBoV detection.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.00889-06