Image Analysis Software Based on Color Segmentation for Characterization of Viability and Physiological Activity of Biofilms

The novel image analysis software package bioImage_L was tested to calculate biofilm structural parameters in oral biofilms stained with dual-channel fluorescent markers. By identifying color tonalities in situ, the software independently processed the color subpopulations and characterized the viab...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2009-03, Vol.75 (6), p.1734-1739
Main Author: CHAVEZ DE PAZ, Luis E
Format: Article
Language:English
Subjects:
Biofilms
Biological and medical sciences
Color
Fluorescence
Fundamental and applied biological sciences. Psychology
Image processing systems
Image Processing, Computer-Assisted - methods
Metabolism
Methods
Microbial Viability
Microbiology
Mouth - microbiology
Software
Software packages
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Description
Summary:The novel image analysis software package bioImage_L was tested to calculate biofilm structural parameters in oral biofilms stained with dual-channel fluorescent markers. By identifying color tonalities in situ, the software independently processed the color subpopulations and characterized the viability and metabolic activity of biofilms.
ISSN:0099-2240
1098-5336
1098-5336
1098-6596
DOI:10.1128/AEM.02000-08