Phosphite induces expression of a putative proteophosphoglycan gene in Phytophthora cinnamomi

The phosphate analogue phosphite is widely used to control diseases of plants caused by oomycete pathogens such as those within the genus Phytophthora . Phosphite inhibits zoospore production and growth of P. cinnamomi . However, very little is known about the underlying mechanism of action. In the...

Full description

Saved in:
Bibliographic Details
Published in:Australasian plant pathology 2009-01, Vol.38 (3), p.235-241
Main Authors: Wong, Mee-Hua, McComb, Jennifer, Hardy, Giles E. St J, Brien, Philip A. O
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
DDRT-PCR
Ecology
Entomology
Life Sciences
Oomycetes
phosphite
Phytophthora
Phytophthora cinnamomi
Plant Pathology
Plant Sciences
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The phosphate analogue phosphite is widely used to control diseases of plants caused by oomycete pathogens such as those within the genus Phytophthora . Phosphite inhibits zoospore production and growth of P. cinnamomi . However, very little is known about the underlying mechanism of action. In the present study, we grew P. cinnamomi in Ribero’s liquid medium with 0.1 mM phosphate, with and without 5 µg phosphite/mL, and used differential display reverse transcriptase–PCR (DDRT–PCR) to identify P. cinnamomi genes that are transcriptionally repressed or induced by phosphite. By using this technique, four differentially expressed bands were identified. However, quantitative measurement of the amount of mRNA transcript by RT–PCR revealed that only one gene was actually phosphite inducible. On the basis of the homology of the deduced amino acid sequence, this gene encodes a proteophosphoglycan. The remaining three bands did not show differential expression.
ISSN:0815-3191
1448-6032
DOI:10.1071/AP08101