Molecular Identification of Saccharum spp. x Erianthus fulvus Hybrids Using Sequence-Characterized Amplified Region Markers

Identification of hybrids derived from crosses between cultivated sugarcane (Saccharum spp.) and its wild relatives, including Erianthus fulvus Nees ex Hack., is important for the development of new sugarcane cultivars. The aim of the present study was to identify true hybrids in 73 F1 progeny obtai...

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Bibliographic Details
Published in:Crop science 2009-05, Vol.49 (3), p.864-870
Main Authors: Wang, Xian-Hong, Yang, Qing-Hui, Li, Fu-Sheng, He, Li-Lian, He, Shun-Chang
Format: Article
Language:English
Subjects:
cultivars
genetic markers
hybrids
interspecific hybridization
molecular sequence data
nucleotide sequences
polymerase chain reaction
random amplified polymorphic DNA technique
Saccharum
sequence characterized amplified regions
sugarcane
wild relatives
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Summary:Identification of hybrids derived from crosses between cultivated sugarcane (Saccharum spp.) and its wild relatives, including Erianthus fulvus Nees ex Hack., is important for the development of new sugarcane cultivars. The aim of the present study was to identify true hybrids in 73 F1 progeny obtained from five crosses with cultivated sugarcane as female parents and E. fulvus as male parents. Three random 10mers were used as primers in random amplified polymorphic DNA (RAPD) reactions. A total of 10 E. fulvus-specific RAPD markers were cloned and sequenced, of which four were successfully applied as sequence-characterized amplified region (SCAR) markers. Based on the four RAPD marker sequences, five SCAR primer pairs were designed for polymerase chain reaction amplifications. Of the 73 progeny examined, 58 produced E. fulvus-specific SCAR bands, thus representing true hybrids of cultivated sugarcane and E. fulvus. These results demonstrate the first use of SCAR marker analysis as an effective tool for the identification of intergeneric sugarcane hybrids.
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci2008.04.0241