effects of palm kernel cake based diet on spermatogenesis in MalinxSanta-Ines rams

• Published in: Animal reproduction science 2009, Vol.115 (1-4), p.182-188
• Main Authors: Yaakub, H, Masnindah, M, Shanthi, G, Sukardi, S, Alimon, A.R
• Format: Article
• Language: English
• Subjects: animal nutrition; bioaccumulation; copper; crossbreds; diet; health status; male fertility; Malin (sheep breed); molybdenum; nutritional status; palm kernel meal; rams; risk assessment; Santa-Ines (sheep breed); Sertoli cells; sheep breeds; sheep feeding; spermatogenesis; spermatozoa; sulfur; testes; toxicity
• ISSN: 0378-4320; 1873-2232

Testes from nine male MalinxSanta-Ines rams with an average body weight of 43.1±3.53kg, were used to study the effects of palm kernel cake (PKC) based diet on spermatogenic cells and to assess copper (Cu) levels in liver, testis and plasma in sheep. Animals were divided into three groups and randomly assigned three dietary treatments using restricted randomization of body weight in completely randomized design. The dietary treatments were 60% palm kernel cake plus 40% oil palm frond (PKC), 60% palm kernel cake plus 40% oil palm frond supplemented with 23mg/kg dry matter of molybdenum as ammonium molybdate [(NH₄)₆Mo₇O₂₄·4H₂O] and 600mg/kg dry matter of sulphate as sodium sulphate [Na₂SO₄] (PKC-MS) and 60% concentrate of corn-soybean mix + 40% oil palm frond (Control), the concentrate was mixed in a ratio of 79% corn, 20% soybean meal and 1% standard mineral mix. The results obtained showed that the number of spermatogonia, spermatocytes, spermatids and Leydig cells were not significantly different among the three treatment groups. However, spermatozoa, Sertoli cells and degenerated cells showed significant changes, which, may be probably due to the Cu content in PKC. Liver and testis Cu levels in the rams under PKC diet was found to be significantly higher (P