High-level expression of the tick-borne encephalitis virus NS1 protaein by using an adenovirus-based vector: protection elicited in a murine model

Tick-borne encephalitis virus (TBEV) encodes an abundant, highly immunogenic nonstructural glycoprotein, NS1. The function of this protein has yet to be determined. We have cloned the NS1 gene from the Neudorfl strain of TBEV under the control of the powerful constitutive cytomegalovirus major immed...

Full description

Saved in:
Bibliographic Details
Published in:Journal of virology 1992, Vol.66 (4), p.2086-2095
Main Authors: Jacobs, S.C, Stephenson, J.R, Wilkinson, G.W.G
Format: Article
Language:English
Subjects:
Adenoviridae
adenovirus recombinant
gene expression
genes
genetic recombination
glycoproteins
immune response
Tick-borne encephalitis virus
translocation signal sequence
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tick-borne encephalitis virus (TBEV) encodes an abundant, highly immunogenic nonstructural glycoprotein, NS1. The function of this protein has yet to be determined. We have cloned the NS1 gene from the Neudorfl strain of TBEV under the control of the powerful constitutive cytomegalovirus major immediate-early promoter into an adenovirus E1 deletion mutant. The novel combination of the cytomegalovirus immediate-early promoter and the adenovirus vector produced extremely high levels of NS1 expression in cells which do not support replication of the adenovirus deletion mutant. The recombinant protein was shown to be indistinguishable from authentic TBEV NS1 in its (i) apparent molecular weight by polyacrylamide gel electrophoresis, (ii) glycosylation pattern, (iii) ability to form high-molecular-weight complexes, and (iv) ability to be secreted from cells. Appropriate processing of NS1 expressed by the adenovirus recombinant occurred independently of any additional TBEV-encoded gene function. When directly inoculated into mice, the recombinant adenovirus RAd51 was shown to elicit an antibody response to the TBEV NS1 protein. immunization with Rad51 conferred protection against challenge with TBEV.
ISSN:0022-538X
1098-5514