Toxicity to Chrysomela tremulae (Coleoptera: Chrysomelidae) of transgenic populars expressing a cysteine proteinase inhibitor

The aim of this study was to test the potential of proteinase inhibitors to control Chrysomela tremulae, a beetle that causes severe damage in young plantations and in short-rotation intensive culture (SRIC) of poplar. As a first step, cysteine proteinases were determined to be the major digestive p...

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Bibliographic Details
Published in:Molecular breeding 1995, Vol.1 (4), p.319-328
Main Authors: Leple, J.C, Bonade-Bottino, M, Augustin, S, Pilate, G, Le Tan, V.D, Delplanque, A, Cornu, D, Jouanin, L
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
bioassays
Chrysomela
cysteine proteinases
digestive system
enzyme activity
gene expression
gene transfer
genetic transformation
hybrids
insecticidal properties
interspecific hybridization
larvae
leaves
messenger RNA
Oryza sativa
oryzacystatin
Populus tremula
Populus tremuloides
proteinase inhibitors
structural genes
toxicity
transgenic plants
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Summary:The aim of this study was to test the potential of proteinase inhibitors to control Chrysomela tremulae, a beetle that causes severe damage in young plantations and in short-rotation intensive culture (SRIC) of poplar. As a first step, cysteine proteinases were determined to be the major digestive proteinases of C. tremulae and oryzacystatin OCI, a cysteine proteinase inhibitor, was shown to inhibit this activity in vitro. The gene encoding OCI was introduced into poplar (Populus tremula x P. tremuloides) and transgenic plants expressing OCI at a high level were selected. Feeding tests on these transgenic plants demonstrate the toxicity of OCI-producing poplar leaves against C. tremulae larvae.
ISSN:1380-3743
1572-9788