Extraction, polymerase chain reaction, and sequencing of a 4 base pair region of the mitochondrial cytochrome oxidase I gene from two species of acetone-preserved damselflies (Odonata: Coenagrionidae, Agrionidae)

Preserved insects are an important data source for many molecular systematics projects. This study investigates the use of acetone-preserved specimens in molecular DNA research. Two species of damselflies, Enallagma civile (Hagen) and Hetaerina americana (F.), were soaked in acetone before drying. T...

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Bibliographic Details
Published in:Environmental entomology 1999, Vol.28 (2), p.143-147
Main Author: Logan, J.A
Format: Article
Language:English
Subjects:
acetone
amino acid sequences
chemical preservation
cytochrome-c oxidase
dna extraction
enallegma civile
extraction
genbank/af072909
genbank/af072910
genes
hetaerina americana
mitochondrial DNA
molecular sequence data
nucleotide sequences
Odonata
polymerase chain reaction
structural genes
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Summary:Preserved insects are an important data source for many molecular systematics projects. This study investigates the use of acetone-preserved specimens in molecular DNA research. Two species of damselflies, Enallagma civile (Hagen) and Hetaerina americana (F.), were soaked in acetone before drying. Total genomic DNA was successfully extracted, amplified, and sequenced from the acetone-preserved damselflies with no noticeable effect from either the acetone or preservation time. Nucleotide sequences of a 440 bp region of the mitochondrial cytochrome oxidase I gene are presented for E. civile and H. americana. These 2 species have reached a saturated divergence level and it seems that the COI gene will not be useful for developing phylogenies at this taxonomic level.
ISSN:0046-225X
1938-2936