Genotype variation in anther culture and effect of ovary coculture in durum wheat

The response of anthers to in vitro culture and the effect of coculture of ovaries on anther culturability have been studied in responsive and recalcitrant cultivars of durum wheat (Triticum turgidum ssp. durum) from Morocco and ICARDA. A large genotypic-dependence of anther culture has been shown i...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 1999, Vol.59 (1), p.71-76
Main Authors: Aiti, F.J, Benlhabib, O, Sharma, H.C, El Jaafari, S, El Hadrami, I
Format: Article
Language:English
Subjects:
androgenesis
anther culture
cultivars
culture media
genetic variation
genotype
gynoecium
regenerative ability
tissue culture
Triticum turgidum subsp. durum
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Summary:The response of anthers to in vitro culture and the effect of coculture of ovaries on anther culturability have been studied in responsive and recalcitrant cultivars of durum wheat (Triticum turgidum ssp. durum) from Morocco and ICARDA. A large genotypic-dependence of anther culture has been shown in 18 cultivars. Their response in term of callus and embryo induction varied from 0 to 13%. Coculture of ovaries with anthers enhanced the response of the most responsive genotype (cv. Sarif) and removed the recalcitrance in Cocorit and Isly cultivars. However, there was no effect of anther-ovary coculture on green plant regeneration. The implication of the genome and the media conditioning by the ovaries on anther response is discussed.
ISSN:0167-6857
1573-5044