Contribution of the ATP-Dependent Protease ClpCP to the Autolysis and Virulence of Streptococcus pneumoniae

The ATP-dependent caseinolytic proteases (Clp) are fundamental for stress tolerance and virulence in many pathogenic bacteria. The role of ClpC in the autolysis and virulence of Streptococcus pneumoniae is controversial. In this study, we tested the role of ClpC in a number of S. pneumoniae strains...

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Bibliographic Details
Published in:Infection and Immunity 2005-02, Vol.73 (2), p.730-740
Main Authors: Ibrahim, Yasser Musa, Kerr, Alison R, Silva, Nuno A, Mitchell, Tim J
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
animal models
Animals
autolysis
bacteria
Bacterial Proteins - metabolism
Bacteriology
Bacteriolysis - genetics
Bacteriolysis - physiology
Biological and medical sciences
blood
Blood - microbiology
disease models
Disease Models, Animal
Endopeptidase Clp - genetics
Endopeptidase Clp - physiology
Fundamental and applied biological sciences. Psychology
heat tolerance
Hot Temperature
Lung - microbiology
lungs
Mice
Microbiology
Miscellaneous
Molecular Pathogenesis
N-Acetylmuramoyl-L-alanine Amidase - metabolism
oxidative stress
Oxidative Stress - physiology
Pneumonia, Pneumococcal - enzymology
Pneumonia, Pneumococcal - metabolism
proteinases
serotypes
Streptococcus pneumoniae
Streptococcus pneumoniae - enzymology
Streptococcus pneumoniae - genetics
Streptococcus pneumoniae - pathogenicity
Streptococcus pneumoniae - physiology
Streptolysins - metabolism
stress tolerance
temperature
virulence
Virulence - genetics
Virulence - physiology
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Summary:The ATP-dependent caseinolytic proteases (Clp) are fundamental for stress tolerance and virulence in many pathogenic bacteria. The role of ClpC in the autolysis and virulence of Streptococcus pneumoniae is controversial. In this study, we tested the role of ClpC in a number of S. pneumoniae strains and found that the contribution of ClpC to autolysis is strain dependent. ClpC is required for the release of autolysin A and pneumolysin in serotype 2 S. pneumoniae strain D39. In vivo, ClpC is required for the growth of the pneumococcus in the lungs and blood in a murine model of disease, but it does not affect the overall outcome of pneumococcal disease. We also report the requirement of ClpP for the growth at elevated temperature and virulence of serotype 4 strain TIGR4 and confirm its contribution to the thermotolerance, oxidative stress resistance, and virulence of D39.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.73.2.730-740.2005