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Characterisation of metal-binding biomolecules in the clam Chamelea gallina by bidimensional liquid chromatography with in series UV and ICP-MS detection
The presence of metal-binding biomolecules has been studied in Chamelea gallina, a bioindicator used for environmental contamination monitoring and very popular for human consumption in the Atlantic southwest coast of Spain. This area is affected by metal pollution from mining activities, which can...
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| Published in: | International journal of environmental analytical chemistry 2011-11, Vol.91 (13), p.1282-1295 |
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| Main Authors: | , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c424t-34cb5750a0729423c7de99ab2fa288276f0e8bd56e4d752a99bfe1976e8643953 |
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| cites | cdi_FETCH-LOGICAL-c424t-34cb5750a0729423c7de99ab2fa288276f0e8bd56e4d752a99bfe1976e8643953 |
| container_end_page | 1295 |
| container_issue | 13 |
| container_start_page | 1282 |
| container_title | International journal of environmental analytical chemistry |
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| creator | Vitoulova, Eva Garcia-Barrera, Tamara Gomez-Ariza, Jose Luis Fisera, Miroslav |
| description | The presence of metal-binding biomolecules has been studied in Chamelea gallina, a bioindicator used for environmental contamination monitoring and very popular for human consumption in the Atlantic southwest coast of Spain. This area is affected by metal pollution from mining activities, which can modify biomolecules expression in this bivalve. Total content of elements was determined by ICP-MS and revealed a remarked presence of Fe, Zn, Cu, As and Mn. A metallomics approach has been optimised for this mollusc using size-exclusion chromatography on column Superdex 30 pg HiLoad 26/60 with in series UV and ICP-MS detection. At least four fractions with molecular weight in the range 1540 to 415 Da were observed with UV detection, but the ICP-MS chromatogram showed the presence of metals of interest only in the first two fractions. The apparent molecular weights of these metal-containing fractions were from 1325 to 764 Da. The fractions containing metals compounds were collected and lyophilised for further purification of reconstituted extracts with a second orthogonal chromatographic separation using reverse phase (RP) HPLC with ICP-MS detection. Several peaks were obtained in this second dimension separation which allows the isolation of As-, Cu- and Zn-containing biomolecules. |
| doi_str_mv | 10.1080/03067319.2010.511204 |
| format | article |
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This area is affected by metal pollution from mining activities, which can modify biomolecules expression in this bivalve. Total content of elements was determined by ICP-MS and revealed a remarked presence of Fe, Zn, Cu, As and Mn. A metallomics approach has been optimised for this mollusc using size-exclusion chromatography on column Superdex 30 pg HiLoad 26/60 with in series UV and ICP-MS detection. At least four fractions with molecular weight in the range 1540 to 415 Da were observed with UV detection, but the ICP-MS chromatogram showed the presence of metals of interest only in the first two fractions. The apparent molecular weights of these metal-containing fractions were from 1325 to 764 Da. The fractions containing metals compounds were collected and lyophilised for further purification of reconstituted extracts with a second orthogonal chromatographic separation using reverse phase (RP) HPLC with ICP-MS detection. 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This area is affected by metal pollution from mining activities, which can modify biomolecules expression in this bivalve. Total content of elements was determined by ICP-MS and revealed a remarked presence of Fe, Zn, Cu, As and Mn. A metallomics approach has been optimised for this mollusc using size-exclusion chromatography on column Superdex 30 pg HiLoad 26/60 with in series UV and ICP-MS detection. At least four fractions with molecular weight in the range 1540 to 415 Da were observed with UV detection, but the ICP-MS chromatogram showed the presence of metals of interest only in the first two fractions. The apparent molecular weights of these metal-containing fractions were from 1325 to 764 Da. The fractions containing metals compounds were collected and lyophilised for further purification of reconstituted extracts with a second orthogonal chromatographic separation using reverse phase (RP) HPLC with ICP-MS detection. Several peaks were obtained in this second dimension separation which allows the isolation of As-, Cu- and Zn-containing biomolecules.</description><subject>Analytical chemistry</subject><subject>arsenic</subject><subject>biochemical compounds</subject><subject>Biomolecules</subject><subject>Bivalvia</subject><subject>Chamelea</subject><subject>Chamelea gallina</subject><subject>Chemical elements</subject><subject>Chromatography</subject><subject>clams</subject><subject>Contamination</subject><subject>Copper</subject><subject>freeze drying</subject><subject>high performance liquid chromatography</subject><subject>humans</subject><subject>indicator species</subject><subject>inductively coupled plasma mass spectrometry</subject><subject>iron</subject><subject>Liquid chromatography</subject><subject>manganese</subject><subject>Marine</subject><subject>metallomics</subject><subject>Metals</subject><subject>mining</subject><subject>Molecular weight</subject><subject>Mollusks</subject><subject>monitoring</subject><subject>pollution</subject><subject>reversed phase high performance liquid chromatography</subject><subject>Separation</subject><subject>size exclusion chromatography</subject><subject>Zinc</subject><issn>1029-0397</issn><issn>0306-7319</issn><issn>1029-0397</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkl9r1TAYh4soOKffQDB45U1n_rVJrkQOUwcThXm8DW_T9DQjbc6SlHE-it_WlE4Y3uwqIfx-zxvypKreEnxBsMQfMcOtYERdUFyOGkIo5s-qM4KpqjFT4vmj_cvqVUq3GBPWSHZW_dmNEMFkG12C7MKMwoAmm8HXnZt7Nx9Q58IUvDWLtwm5GeXRIuNhQqU6WW8BHcB7NwPqTiXcu8nOqZDAI-_uFtcjM8YwQQ6HCMfxhO5dHldQKkMLcv8bwdyjq93P-vsN6m22Zr3I6-rFAD7ZNw_rebX_cvlr962-_vH1avf5ujac8lwzbrpGNBiwoIpTZkRvlYKODkClpKIdsJVd37SW96KhoFQ3WKJEa2XLmWrYefVh4x5juFtsynpyyVjvYbZhSZq0nFIhpGqfjmIsJROqoSX6_r_obVhieZOkVXFBZMPX0XwLmRhSinbQx-gmiKdC0qtZ_c-sXs3qzWypfdpqbh5CnOA-RN_rDCcf4hBhNi5p9gTh3UYYIGg4FPd6f1MCvPwLgTlp2V_ZhLOW</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Vitoulova, Eva</creator><creator>Garcia-Barrera, Tamara</creator><creator>Gomez-Ariza, Jose Luis</creator><creator>Fisera, Miroslav</creator><general>Taylor & Francis Group</general><general>Taylor & Francis LLC</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7SN</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>SOI</scope><scope>7TV</scope><scope>H95</scope><scope>7SU</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>20111101</creationdate><title>Characterisation of metal-binding biomolecules in the clam Chamelea gallina by bidimensional liquid chromatography with in series UV and ICP-MS detection</title><author>Vitoulova, Eva ; Garcia-Barrera, Tamara ; Gomez-Ariza, Jose Luis ; Fisera, Miroslav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-34cb5750a0729423c7de99ab2fa288276f0e8bd56e4d752a99bfe1976e8643953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical chemistry</topic><topic>arsenic</topic><topic>biochemical compounds</topic><topic>Biomolecules</topic><topic>Bivalvia</topic><topic>Chamelea</topic><topic>Chamelea gallina</topic><topic>Chemical elements</topic><topic>Chromatography</topic><topic>clams</topic><topic>Contamination</topic><topic>Copper</topic><topic>freeze drying</topic><topic>high performance liquid chromatography</topic><topic>humans</topic><topic>indicator species</topic><topic>inductively coupled plasma mass spectrometry</topic><topic>iron</topic><topic>Liquid chromatography</topic><topic>manganese</topic><topic>Marine</topic><topic>metallomics</topic><topic>Metals</topic><topic>mining</topic><topic>Molecular weight</topic><topic>Mollusks</topic><topic>monitoring</topic><topic>pollution</topic><topic>reversed phase high performance liquid chromatography</topic><topic>Separation</topic><topic>size exclusion chromatography</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vitoulova, Eva</creatorcontrib><creatorcontrib>Garcia-Barrera, Tamara</creatorcontrib><creatorcontrib>Gomez-Ariza, Jose Luis</creatorcontrib><creatorcontrib>Fisera, Miroslav</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Environmental Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>International journal of environmental analytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vitoulova, Eva</au><au>Garcia-Barrera, Tamara</au><au>Gomez-Ariza, Jose Luis</au><au>Fisera, Miroslav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of metal-binding biomolecules in the clam Chamelea gallina by bidimensional liquid chromatography with in series UV and ICP-MS detection</atitle><jtitle>International journal of environmental analytical chemistry</jtitle><date>2011-11-01</date><risdate>2011</risdate><volume>91</volume><issue>13</issue><spage>1282</spage><epage>1295</epage><pages>1282-1295</pages><issn>1029-0397</issn><issn>0306-7319</issn><eissn>1029-0397</eissn><abstract>The presence of metal-binding biomolecules has been studied in Chamelea gallina, a bioindicator used for environmental contamination monitoring and very popular for human consumption in the Atlantic southwest coast of Spain. This area is affected by metal pollution from mining activities, which can modify biomolecules expression in this bivalve. Total content of elements was determined by ICP-MS and revealed a remarked presence of Fe, Zn, Cu, As and Mn. A metallomics approach has been optimised for this mollusc using size-exclusion chromatography on column Superdex 30 pg HiLoad 26/60 with in series UV and ICP-MS detection. At least four fractions with molecular weight in the range 1540 to 415 Da were observed with UV detection, but the ICP-MS chromatogram showed the presence of metals of interest only in the first two fractions. The apparent molecular weights of these metal-containing fractions were from 1325 to 764 Da. The fractions containing metals compounds were collected and lyophilised for further purification of reconstituted extracts with a second orthogonal chromatographic separation using reverse phase (RP) HPLC with ICP-MS detection. Several peaks were obtained in this second dimension separation which allows the isolation of As-, Cu- and Zn-containing biomolecules.</abstract><cop>Abingdon</cop><pub>Taylor & Francis Group</pub><doi>10.1080/03067319.2010.511204</doi><tpages>14</tpages></addata></record> |
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| ispartof | International journal of environmental analytical chemistry, 2011-11, Vol.91 (13), p.1282-1295 |
| issn | 1029-0397 0306-7319 1029-0397 |
| language | eng |
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| source | Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Science and Technology Collection (Reading list) |
| subjects | Analytical chemistry arsenic biochemical compounds Biomolecules Bivalvia Chamelea Chamelea gallina Chemical elements Chromatography clams Contamination Copper freeze drying high performance liquid chromatography humans indicator species inductively coupled plasma mass spectrometry iron Liquid chromatography manganese Marine metallomics Metals mining Molecular weight Mollusks monitoring pollution reversed phase high performance liquid chromatography Separation size exclusion chromatography Zinc |
| title | Characterisation of metal-binding biomolecules in the clam Chamelea gallina by bidimensional liquid chromatography with in series UV and ICP-MS detection |
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