SSR-Based DNA Barcodes as a Tool for Identification of Eggplant Genotypes

Eggplant (Solanum melongena L.) is an important crop in terms of economic and genetic significance in tropical and subtropical regions. The Indian Institute of Horticulture Research (IIHR) eggplant breeding program produced five genotypes with increased yield and excellent producer acceptance. Molec...

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Bibliographic Details
Published in:International journal of vegetable science 2012-07, Vol.18 (3), p.260-271
Main Authors: Chinnappareddy, Lakshmana Reddy Dhoranalapalli, Khandagale, Kiran, Srinivas Reddy, Srikanth Hongasandra, Kanupriya, C, Chennareddy, Aswath, Singh, T. H
Format: Article
Language:English
Subjects:
alleles
breeding
Deoxyribonucleic acid
DNA
DNA barcode
DNA barcoding
eggplants
heterozygosity
loci
microsatellite repeats
Microsatellites
Polymorphism
probability
Solanum melongena
subtropics
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Summary:Eggplant (Solanum melongena L.) is an important crop in terms of economic and genetic significance in tropical and subtropical regions. The Indian Institute of Horticulture Research (IIHR) eggplant breeding program produced five genotypes with increased yield and excellent producer acceptance. Molecular profiles of these genotypes were developed using 39 EST-SSR primers for maximum discrimination and repeatability at 35 loci. In total there were 181 alleles with the number of alleles per locus ranging from 9.0 (EEMS 24) to 3 (EEMS 46 and EEMS 20), with a mean of 5.02 alleles per locus ranging from 85 to 300 bp. Polymorphic information content of the markers ranged from 0.343 to 0.794. Expected heterozygosity ranged from 0.560 to 0.880 and the probability of identity ranged from 0.010 to 0.376. Pair-wise comparison of microsatellite data led to development of an unweighted pair group method with arithmetic mean dendrogram and DNA barcodes for easy and accurate identification of eggplant genotypes with combinations of their morphological traits. The results indicate that ‘IIHR-3’ and ‘IIHR-7’ originated from a single source and ‘Arka Anand’ is a cross between ‘IIHR-3’ and ‘SM-6-6’, indicating a good fit with genetic similarity values. The DNA barcode fingerprint can be deposited and used for clear identification of eggplant genotypes and to address intellectual property rights–related issues.
ISSN:1931-5279
1931-5260
1931-5279
DOI:10.1080/19315260.2011.633976