Impact of replacing the chloroplast agglutination test with enzyme-linked immunosorbent assay (ELISA) on the health status and productivity of seed potatoes

The impact of replacing the chloroplast agglutination test with enzyme-linked immunosorbent assay (ELISA) was studied on the health status and productivity of different stages of nucleus seed potato. The study revealed that introduction of ELISA in place of chloroplast agglutination test of Stage I...

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Bibliographic Details
Published in:Archiv für Phytopathologie und Pflanzenschutz 2011-12, Vol.44 (20), p.1999-2003
Main Authors: Sharma, Ashwani K, Venkatasalam, E. P
Format: Article
Language:English
Subjects:
agglutination tests
chloroplast agglutination test
Chloroplasts
clones
ELISA
enzyme-linked immunosorbent assay
health status
Immunoassay
indexing
nucleus
planting
seed
seed productivity
seed tubers
Solanum tuberosum
testing
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Summary:The impact of replacing the chloroplast agglutination test with enzyme-linked immunosorbent assay (ELISA) was studied on the health status and productivity of different stages of nucleus seed potato. The study revealed that introduction of ELISA in place of chloroplast agglutination test of Stage I plants resulted in a significant improvement of the overall health status of nucleus seed potatoes. After the replacement, health standards during ELISA of indexed tubers (for next year planting) improved from 80.3 to 95.9%, whereas, some improvement in the health status of Stage II clones (99.5–99.9%) was also noticed. Following ELISA, per cent incidence of mosaics during field roguings in Stages I, II and III was also reduced from 1.74, 0.92 and 0.39% to 1.55, 0.67 and 0.29%, respectively and the corresponding increase in yield were 1.3, 11.0 and 7.0%, respectively.
ISSN:1477-2906
0323-5408
1477-2906
DOI:10.1080/03235408.2011.559039