Phosphodiesterase DipA (PA5017) Is Essential for Pseudomonas aeruginosa Biofilm Dispersion

• Published in: Journal of Bacteriology 2012-06, Vol.194 (11), p.2904-2915
• Main Authors: Roy, Ankita Basu, Petrova, Olga E, Sauer, Karin
• Format: Article
• Language: English
• Subjects: ammonium chloride; bacterial motility; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biochemistry; biofilm; Biofilms; Biological and medical sciences; Biosynthesis; Cell Membrane - enzymology; Cell Membrane - genetics; Cell Membrane - metabolism; Cyclic GMP - metabolism; Enzymes; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; genes; glutamic acid; Gram-negative bacteria; in vivo studies; mercuric chloride; Microbiology; Miscellaneous; mutants; Mutation; nitric oxide; nutrient content; Phosphoric Diester Hydrolases - chemistry; Phosphoric Diester Hydrolases - genetics; Phosphoric Diester Hydrolases - metabolism; Protein Structure, Tertiary; protein synthesis; Protein Transport; Pseudomonas aeruginosa; Pseudomonas aeruginosa - chemistry; Pseudomonas aeruginosa - enzymology; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - physiology; screening
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.05346-11

Although little is known regarding the mechanism of biofilm dispersion, it is becoming clear that this process coincides with alteration of cyclic di-GMP (c-di-GMP) levels. Here, we demonstrate that dispersion by Pseudomonas aeruginosa in response to sudden changes in nutrient concentrations resulted in increased phosphodiesterase activity and reduction of c-di-GMP levels compared to biofilm and planktonic cells. By screening mutants inactivated in genes encoding EAL domains for nutrient-induced dispersion, we identified in addition to the previously reported ΔrbdA mutant a second mutant, the ΔdipA strain (PA5017 [dispersion-induced phosphodiesterase A]), to be dispersion deficient in response to glutamate, nitric oxide, ammonium chloride, and mercury chloride. Using biochemical and in vivo studies, we show that DipA associates with the membrane and exhibits phosphodiesterase activity but no detectable diguanylate cyclase activity. Consistent with these data, a ΔdipA mutant exhibited reduced swarming motility, increased initial attachment, and polysaccharide production but only somewhat increased biofilm formation and c-di-GMP levels. DipA harbors an N-terminal GAF (cGMP-specific phosphodiesterases, adenylyl cyclases, and FhlA) domain and two EAL motifs within or near the C-terminal EAL domain. Mutational analyses of the two EAL motifs of DipA suggest that both are important for the observed phosphodiesterase activity and dispersion, while the GAF domain modulated DipA function both in vivo and in vitro without being required for phosphodiesterase activity. Dispersion was found to require protein synthesis and resulted in increased dipA expression and reduction of c-di-GMP levels. We propose a role of DipA in enabling dispersion in P. aeruginosa biofilms.