Chemical and Biological Activities of Haplophyllum tuberculatum Organic Extracts and Essential Oil

Chemical composition, antimicrobial activity and antioxidative proprieties were assessed in air-dried shoots of Haplophyllum tuberculatum . Samples were subjected either to organic fractionated extraction (hexane, chloroform and methanol) or hydrodistillation for essential oil extraction. We found t...

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Bibliographic Details
Published in:Journal of essential oil-bearing plants (Dehra Dun) 2014-09, Vol.17 (5), p.787-796
Main Authors: Debouba, Mohamed, Khemakhem, Bassem, Zouari, Sami, Meskine, Abir, Gouia, Houda
Format: Article
Language:English
Subjects:
air drying
antibacterial properties
antimicrobial activity
antioxidant activity
Candida albicans
chemical composition
chloroform
essential oil
essential oils
free radical scavengers
gas chromatography
Gram-negative bacteria
H. tuberculatum
Haplophyllum tuberculatum
hexane
mass spectrometry
methanol
oils
oleic acid
organic extract
palmitic acid
polyphenols
shoots
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Summary:Chemical composition, antimicrobial activity and antioxidative proprieties were assessed in air-dried shoots of Haplophyllum tuberculatum . Samples were subjected either to organic fractionated extraction (hexane, chloroform and methanol) or hydrodistillation for essential oil extraction. We found that methanol extract (ME) was the most enriched in polyphenols and that flavonoïds were more concentrated in chloroform one (CE). Antimicrobial tests showed that organic extracts were more effective against Gram negative bacteria than Gram positive ones. The best antibacterial activity was exhibited by ME which was also active against Candida albicans . Gas chromatography/mass spectrometry analysis of extracted essential oil from air-dried shoots revealed 18 indentified components representing 93.46 % of total oil. The major components were hexadecanoic acid (40.21 %) and oleic acid (26.75 %). Antioxidative assays showed that H. tuberculatum essential oil exhibited a weak ferric reducing power and moderate free radical scavenging activity. Obtained data argued that H. tuberculatum shoots are sources of natural products of promising antimicrobial and antioxidative capacities.
ISSN:0976-5026
0972-060X
0976-5026
DOI:10.1080/0972060X.2014.958545