Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis

The spermatogonial stem-cell renewal factor (SSRF) was named since its function in spermatogonial mitosis was reported in Japanese eel. Our previous study showed that a homolog of SSRF was highly expressed in the ovary of triploid shrimp, but not expressed in the ovary of diploid shrimp. To understa...

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Bibliographic Details
Published in:Invertebrate reproduction & development 2014-07, Vol.58 (3), p.226-234
Main Authors: Wen, Rong, Li, Fuhua, Xie, Yusu, Li, Shihao, Zhang, Chengsong, Yu, Kuijie, Xiang, Jianhai
Format: Article
Language:English
Subjects:
amino acids
Anguilla japonica
animal ovaries
complementary DNA
development
diploidy
Fenneropenaeus chinensis
genes
hepatopancreas
humans
immunohistochemistry
juveniles
messenger RNA
mitosis
nauplii
nerve tissue
open reading frames
ovary
penaeid shrimp
postlarvae
reverse transcriptase polymerase chain reaction
shrimp
stomach
testes
testis
triploid
triploidy
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Summary:The spermatogonial stem-cell renewal factor (SSRF) was named since its function in spermatogonial mitosis was reported in Japanese eel. Our previous study showed that a homolog of SSRF was highly expressed in the ovary of triploid shrimp, but not expressed in the ovary of diploid shrimp. To understand the function of SSRF in shrimp, the full-length cDNA of ssrf gene was cloned from Chinese shrimp Fenneropenaeus chinensis (Fcssrf) and its expression was analyzed. The full length of Fcssrf cDNA was 2588 bp and it contained an open reading frame encoding 450 amino acids. The predicted tertiary structure of FcSSRF was very similar to that of SSRF/eSRS34 from Anguilla japonica and TP/PD-ECGF from Homo sapiens . RT-PCR analysis showed that the Fcssrf was highly expressed in nerve, testis, hepatopancreas, gill, and stomach rather than in ovary. Expression of Fcssrf mRNA was not detected during embryonic stages and larval stages, from the nauplii to the post-larvae stage, in diploid, and triploid shrimp. However, it began to be expressed in juvenile stages (June–September) in diploid and triploid shrimp. Immunohistochemical analyses showed that FcSSRF was identified in both the diploid testis and triploid ovary. We inferred that the Fcssrf might be related to testis development.
ISSN:2157-0272
0792-4259
2157-0272
DOI:10.1080/07924259.2014.905501