Enzymatic Degumming of Silk with Microbial Proteases

Degumming of Chinese bivoltine silk with alkaline proteases from various microbial sources was investigated and compared with commercial enzymes. Among the proteases tested, two fungal and two actinomycete proteases were promising, which showed weight loss similar to conventional method (19.58% to 2...

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Bibliographic Details
Published in:Journal of natural fibers 2013, Vol.10 (2), p.98-111
Main Authors: More, S.V, Khandelwal, H.B, Joseph, M.A, Laxman, R.S
Format: Article
Language:English
Subjects:
alkaline protease
bivoltine
Conidiobolus
degumming
enzymatic treatment
fungi
microbial
proteinases
sericin
silk
silkworms
tensile strength
weight loss
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Summary:Degumming of Chinese bivoltine silk with alkaline proteases from various microbial sources was investigated and compared with commercial enzymes. Among the proteases tested, two fungal and two actinomycete proteases were promising, which showed weight loss similar to conventional method (19.58% to 21.78%). Conidiobolus brefeldianus and BOA-2 proteases were best enzymes, which showed weight loss similar to conventional method with low enzyme concentrations and in shorter time. No significant differences were found in tensile strength or elongation at break by enzymatic degumming indicating that there was no damage to the fiber. Scanning electron micrographs showed the sericin deposits were removed and the fibers were separated.
ISSN:1544-046X
1544-0478
1544-046X
DOI:10.1080/15440478.2012.761114