Analysis of chloramphenicol in honeys of different geographical origin by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

A sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to detect trace amounts of the antibiotic chloramphenicol (CAP) in honey. The methodology entailed a solid-phase extraction of aqueous honey solutions followed by liquid-liquid partitioning, filtration and dir...

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Bibliographic Details
Published in:Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2003-04, Vol.20 (4), p.335-342
Main Authors: Verzegnassi, L, Royer, D, Mottier, P, Stadler, R.H
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - analysis
Antibiotics
Biological and medical sciences
Chloramphenicol
Chloramphenicol - analysis
Chromatography, Liquid - methods
Confectionery products and chocolate industries, honey
drug residues
Electrospray Ionization Liquid Chromatography-tandem Mass Spectrometry
electrospray ionization mass spectrometry
European Union
filtration
food additives
Food Analysis
Food Contamination - analysis
Food industries
Fundamental and applied biological sciences. Psychology
honey
Honey - analysis
ionization
isotope labeling
liquid chromatography
monitoring
provenance
Raw Honey
solid phase extraction
Spectrometry, Mass, Electrospray Ionization - methods
Survey
tandem mass spectrometry
veterinary drugs
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Summary:A sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to detect trace amounts of the antibiotic chloramphenicol (CAP) in honey. The methodology entailed a solid-phase extraction of aqueous honey solutions followed by liquid-liquid partitioning, filtration and direct injection onto the LC-MS/MS system. Honey extracts were spiked with an isotopically labelled internal standard (d5-CAP) to compensate for analyte loss and potential ion suppression during the MS stage. Detection of the analyte was achieved by negative ionization electrospray in the selected reaction monitoring (SAM) mode. For confirmation, four characteristic mass transitions were monitored each for the analyte and the surrogate standard. The method was validated according to the latest European Union criteria for the analyses of veterinary drug residues in food. At all three fortification levels studied (0.1, 0.2, 0.5 μg kg-1) the method was accurate to within 15%. The repeatability and within-laboratory reproducibilities were
ISSN:1944-0049
0265-203X
1944-0057
1464-5122
DOI:10.1080/0265203031000122003