Sirtuin 6 (SIRT6) rescues the decline of homologous recombination repair during replicative senescence

• Published in: Proceedings of the National Academy of Sciences - PNAS 2012-07, Vol.109 (29), p.11800-11805
• Main Authors: Mao, Zhiyong, Tian, Xiao, Van Meter, Michael, Ke, Zhonghe, Gorbunova, Vera, Seluanov, Andrei
• Format: Article
• Language: English
• Subjects: Age; Age Factors; Biological Sciences; Cell cycle; Cell Line; Cell lines; cell senescence; Cells; Cellular senescence; Cellular Senescence - physiology; Chromosomes; Delta cells; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded; DNA repair; Fibroblasts; Flow Cytometry; Fluorescent Antibody Technique; Gene conversion; genome; Genomic Instability; Genomics; homologous recombination; Homologous Recombination - genetics; Homologous Recombination - physiology; Humans; Lymphocytes; middle-aged adults; NAD ADP-ribosyltransferase; neoplasms; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases - metabolism; Polymerase Chain Reaction; Proteins; Sirtuins - metabolism; Tissues; Transfection
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1200583109

Genomic instability is a hallmark of aging tissues. Genomic instability may arise from the inefficient or aberrant function of DNA double-stranded break (DSB) repair. DSBs are repaired by homologous recombination (HR) and nonhomologous DNA end joining (NHEJ). HR is a precise pathway, whereas NHEJ frequently leads to deletions or insertions at the repair site. Here, we used normal human fibroblasts with a chromosomally integrated HR reporter cassette to examine the changes in HR efficiency as cells progress to replicative senescence. We show that HR declines sharply with increasing replicative age, with an up to 38-fold decrease in efficiency in presenescent cells relative to young cells. This decline is not explained by a reduction of the number of cells in S/G ₂/M stage as presenescent cells are actively dividing. Expression of proteins involved in HR such as Rad51, Rad51C, Rad52, NBS1, and Sirtuin 6 (SIRT6) diminished with cellular senescence. Supplementation of Rad51, Rad51C, Rad52, and NBS1 proteins, either individually or in combination, did not rescue the senescence-related decline of HR. However, overexpression of SIRT6 in “middle-aged” and presenescent cells strongly stimulated HR repair, and this effect was dependent on mono-ADP ribosylation activity of poly(ADP-ribose) polymerase (PARP1). These results suggest that in aging cells, the precise HR pathway becomes repressed giving way to a more error-prone NHEJ pathway. These changes in the processing of DSBs may contribute to age-related genomic instability and a higher incidence of cancer with age. SIRT6 activation provides a potential therapeutic strategy to prevent the decline in genome maintenance.