Trisomy 21-associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells

Patients with Down syndrome (trisomy 21, T21) have hematologic abnormalities throughout life. Newborns frequently exhibit abnormal blood counts and a clonal preleukemia. Human T21 fetal livers contain expanded erythro-megakaryocytic precursors with enhanced proliferative capacity. The impact of T21...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2012-10, Vol.109 (43), p.17573-17578
Main Authors: Chou, Stella T, Byrska-Bishop, Marta, Tober, Joanna M, Yao, Yu, VanDorn, Daniel, Opalinska, Joanna B, Mills, Jason A, Choi, John Kim, Speck, Nancy A, Gadue, Paul, Hardison, Ross C, Nemiroff, Richard L, French, Deborah L, Weiss, Mitchell J
Format: Article
Language:English
Subjects:
Biological Sciences
Blood
Cell Differentiation
Down Syndrome
Embryonic stem cells
erythropoiesis
Gene Expression Profiling
Genes
Hematology
Hematopoiesis
Hematopoiesis - genetics
Hematopoietic stem cells
human development
Humans
Induced pluripotent stem cells
Leukemia
Liver
neonates
Pathology
patients
Pluripotent stem cells
Pluripotent Stem Cells - cytology
Progenitor cells
Real-Time Polymerase Chain Reaction
RNA, Messenger - genetics
Stem cells
trisomics
yolk sac
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Summary:Patients with Down syndrome (trisomy 21, T21) have hematologic abnormalities throughout life. Newborns frequently exhibit abnormal blood counts and a clonal preleukemia. Human T21 fetal livers contain expanded erythro-megakaryocytic precursors with enhanced proliferative capacity. The impact of T21 on the earliest stages of embryonic hematopoiesis is unknown and nearly impossible to examine in human subjects. We modeled T21 yolk sac hematopoiesis using human induced pluripotent stem cells (iPSCs). Blood progenitor populations generated from T21 iPSCs were present at normal frequency and proliferated normally. However, their developmental potential was altered with enhanced erythropoiesis and reduced myelopoiesis, but normal megakaryocyte production. These abnormalities overlap with those of T21 fetal livers, but also reflect important differences. Our studies show that T21 confers distinct developmental stage- and species-specific hematopoietic defects. More generally, we illustrate how iPSCs can provide insight into early stages of normal and pathological human development.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1211175109