Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

Xenopus LAP2β protein is the single isoform expressed in XTC cells. The protein localizes on heterochromatin clusters both at the nuclear envelope and inside a cell nucleus. The majority of XLAP2β fraction neither colocalizes with TPX2 protein during interphase nor can be immunoprecipitated with X...

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Bibliographic Details
Published in:Protoplasma 2016, Vol.253 (3), p.943-956
Main Authors: Dubińska-Magiera, Magda, Magdalena Chmielewska, Katarzyna KozioÅ, Magdalena Machowska, Christopher J. Hutchison, Martin W. Goldberg, Ryszard Rzepecki
Format: Article
Language:English
Subjects:
antibodies
artificial cells
cell death
cell viability
heterochromatin
interphase
mitosis
nuclear membrane
nucleoporins
plasmids
protein synthesis
small interfering RNA
Xenopus
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Summary:Xenopus LAP2β protein is the single isoform expressed in XTC cells. The protein localizes on heterochromatin clusters both at the nuclear envelope and inside a cell nucleus. The majority of XLAP2β fraction neither colocalizes with TPX2 protein during interphase nor can be immunoprecipitated with XLAP2β antibody. Knockdown of the XLAP2β protein expression in XTC cells by synthetic siRNA and plasmid encoded siRNA resulted in nuclear abnormalities including changes in shape of nuclei, abnormal chromatin structure, loss of nuclear envelope, mislocalization of integral membrane proteins of INM such as lamin B2, mislocalization of nucleoporins, and cell death. Based on timing of cell death, we suggest mechanism associated with nucleus reassembly or with entry into mitosis. This confirms that Xenopus LAP2 protein is essential for the maintenance of cell nucleus integrity and the process of its reassembly after mitosis.
ISSN:0033-183X
1615-6102