Changes in the activity and subcellular distribution of PPO in 'Delicious' apples during controlled atmosphere storage

Polyphenol oxidase (PPO) activity in 4,000 X g, 100,000 X g, 200 X g and soluble fractions 'Delicious' apples was monitored during 28 weeks of controlled atmosphere storage. PPO activity in 4,000 X g and 100,000 X g fractions decreased, while that in soluble and 200 X g fractions increased...

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Bibliographic Details
Published in:Current microbiology 1991-11, Vol.23 (5)
Main Authors: Barrett, D.M. (Oregon State University, Corvallis, OR), Lee, C.Y, Liu, F.W
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ALMACENAMIENTO EN ATMOSF CONTROL
CATECHOL OXYDASE
CATECOL OXIDASA
MANZANA
POMME
STOCKAGE EN ATMOSPHERE CONTROLEE
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Summary:Polyphenol oxidase (PPO) activity in 4,000 X g, 100,000 X g, 200 X g and soluble fractions 'Delicious' apples was monitored during 28 weeks of controlled atmosphere storage. PPO activity in 4,000 X g and 100,000 X g fractions decreased, while that in soluble and 200 X g fractions increased. These shifts in subcellular location occurred sooner in apples which were stored under high CO2 conditions (2.5-6% O2, 8-12% CO2) than those under normal CA conditions (2% O2, 3% CO2). Polyacrylamide gel electrophoresis of 4,000 X g fractions isolated from 'Delicious' apples at harvest showed 2 bands with PPO activity, while stored samples all had 3 bands. Isoenzyme patterns were found to vary in different subcellular fractions
ISSN:0343-8651
1432-0991