In vitro activation of binitrogenase reductase from the cyanobacterium Anabaena variabilis (ATCC 29413)

Nitrogenase of the heterocystous cyanobacterium Anabaena variabilis was inactivated in vivo (S. Reich, H. Almon, and P. Boger, FEMS Microbiol. Lett. 34:53-56, 1986). Partially purified and modified (inactivated) dinitrogenase reductase (Fe-protein) of such cells was reactivated by isolated membrane...

Full description

Saved in:
Bibliographic Details
Published in:Journal of bacteriology 1992-10, Vol.174 (19)
Main Authors: Bohm, I. (Universitat Konstanz, Konstanz, Germany), Halbherr, A, Smaglinski, S, Ernst, A, Boger, P
Format: Article
Language:English
Subjects:
ACETILENO
ACETYLENE
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ANABAENA
MEMBRANA
MEMBRANE
NITROGENASA
NITROGENASE
OXIDORREDUCTASAS
OXYDOREDUCTASE
REDUCCION
REDUCTION
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nitrogenase of the heterocystous cyanobacterium Anabaena variabilis was inactivated in vivo (S. Reich, H. Almon, and P. Boger, FEMS Microbiol. Lett. 34:53-56, 1986). Partially purified and modified (inactivated) dinitrogenase reductase (Fe-protein) of such cells was reactivated by isolated membrane fractions of A. variabilis or of Rhodospirillum rubrum, and acetylene reduction was measured. Reactivation requires ATP, Mg2+, and Mn2+. The activating principle is localized in the heterocyst and was found effective only when prepared from cells exhibiting active nitrogenase. It also restores the activity of modified Fe-protein from R. rubrum
ISSN:0021-9193
1098-5530